DIABETES AND GLUCOSE TRANSPORTER GENE-EXPRESSION IN RAT SMALL-INTESTINE
DIABETES AND GLUCOSE TRANSPORTER GENE-EXPRESSION IN RAT SMALL-INTESTINE
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DOI：
10.1016/0006-291x(91)92053-m
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发表时间：
1991-12-31
影响因子：
3.1
通讯作者：
HAGIHIRA, H
中科院分区：
文献类型：
--
作者：
MIYAMOTO, K;HASE, K;HAGIHIRA, H
采用RNA印迹法检测了链脲佐菌素（STZ）诱导的糖尿病大鼠小肠钠依赖性葡萄糖转运蛋白（SGLT 1）和5种葡萄糖易化转运蛋白（GLUT 1 -5）基因的表达。SGLT 1 mRNA转录产物的条带为4.5Kb和2.8Kb（非常微弱的条带）。在30和60天STZ大鼠中，SGLT 1 mRNA水平显著增加，但在急性糖尿病大鼠（2至10天STZ大鼠）中无变化。GLUT 2 mRNA水平与D-半乳糖转运活性平行变化，在5天STZ大鼠中增加约4倍。GLUT 5 mRNA转录产物有5.1Kb、2.8Kb和2.0Kb三条带，30和60天STZ组GLUT 5 mRNA表达水平明显降低。这些结果表明，易化葡萄糖转运蛋白（GLUT 2），除了钠离子依赖性葡萄糖转运蛋白（SGLT 1），可能在糖尿病大鼠肠道葡萄糖转运中发挥重要作用。
The expressions of Na+-dependent glucose transporter (SGLT1) and five facilitative glucose transporter genes (GLUT1-5) in the small intestine of streptozotocin (STZ)-induced diabetic rats were examined by RNA blotting analysis. The transcripts of SGLT1 mRNA gave bands of 4.5 Kilobases(Kb) and 2.8Kb (very faint band). The levels of SGLT1 mRNA were significantly increased in 30- and 60-day STZ rats, but not changed in acute diabetic rats (2- to 10- day STZ rats). The GLUT2 mRNA levels changed in parallel with the D-galactose transport activity, being increased about 4-fold in 5-day STZ rats. The transcripts of GLUT5 mRNA gave three bands of 5.1Kb, 2.8Kb and 2.0Kb, whose levels were significantly reduced in 30- and 60-day STZ rats. These results suggest that the facilitative glucose transporter (GLUT2), in addition to the Na+-dependent glucose transporter (SGLT1), may play an important role in intestinal glucose transport in diabetic rats.