E-cadherin is the receptor for internalin, a surface protein required for entry of L-monocytogenes into epithelial cells
E-cadherin is the receptor for internalin, a surface protein required for entry of L-monocytogenes into epithelial cells
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DOI：
10.1016/s0092-8674(00)81070-3
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发表时间：
1996-03-22
期刊：
影响因子：
64.5
通讯作者：
Cossart, P
中科院分区：
文献类型：
--
作者：
Mengaud, J;Ohayon, H;Cossart, P
我们首次发现了一种细胞受体，该受体介导革兰氏阳性细菌进入非吞噬细胞。通过亲和层析的方法，我们确定E-钙粘蛋白是内毒素的配基，内毒素是一种进入上皮细胞所必需的单核细胞增多性李斯特菌蛋白。鸡E-钙粘附素同源物(L-CAM)在转基因成纤维细胞中的表达显著增加了单核细胞增多性乳杆菌的进入，并促进了表达内源性钙粘附素的重组伊诺氏乳杆菌的进入，但不促进野生型非侵袭型无核细胞黏附素或单核细胞增多性乳杆菌内源性缺陷突变体的进入。此外，L-细胞黏附分子特异性抗体可阻断内源性细胞黏附分子的进入。与沙门氏菌不同的是，李斯特菌进入细胞的机制是诱导吞噬作用，而不是膜褶皱。这项工作揭示了E-钙粘蛋白的一种新型的异亲相互作用。
We report the first identification of a cellular receptor mediating entry of a gram-positive bacterium into nonphagocytotic cells. By an affinity chromatography approach, we identified E-cadherin as the ligand for internalin, an L. monocytogenes protein essential for entry into epithelial cells. Expression of the chicken homolog of E-cadherin (L-CAM) in transfected fibroblasts dramatically increases entry of L. monocytogenes and promotes that of a recombinant L. innocua strain expressing internalin but does not promote entry of the wild-type noninvasive L. innocua or that of an internalin-deficient mutant of L. monocytogenes. Furthermore, L-CAM-specific antibodies block internalin-mediated entry. In contrast to Salmonella, Listeria enters cells by a mechanism of induced phagocytosis occurring without membrane ruffling. This work reveals a novel type of heterophilic interactions for E-cadherin.