Functional and physical interaction of yeast Mgs1 with PCNA:: Impact on RAD6-dependent DNA damage tolerance
Functional and physical interaction of yeast Mgs1 with PCNA:: Impact on RAD6-dependent DNA damage tolerance
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DOI：
10.1128/mcb.00307-06
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发表时间：
2006-07-01
影响因子：
5.3
通讯作者：
Shinagawa, Hideo
中科院分区：
文献类型：
--
作者：
Hishida, Takashi;Ohya, Tomoko;Shinagawa, Hideo
增殖细胞核抗原（PCNA）是DNA合成过程中需要的一种滑动钳，它为DNA复制中起作用的各种其他蛋白质提供了附着位点。DNA修复。细胞周期进程和染色质组装。研究表明，泛素或SUMO对PCNA的不同翻译后修饰在控制挽救停滞复制分叉的途径选择方面起着关键作用。在这里，我们探讨了Mgs1和PCNA在复制分叉修复中的作用。我们提供的证据表明，Mgs1与PCNA存在物理关联，并且在没有外源DNA损伤的情况下，Mgs1有助于抑制RAD6 DNA损伤耐受途径。我们还发现，PCNA summoylation抑制mgs1 rad18双突变体的生长，其中PCNA summoylation和Srs2 DNA解旋酶协同阻止rad52依赖性同源重组。mg1的拟议角色。Srs2。和在复制停止期间修饰的PCNA强调了调节RAD6和RAD52途径以避免基因组不稳定的重要性。
Proliferating cell nuclear antigen (PCNA), a sliding clamp required for processive DNA synthesis, provides attachment sites for various other proteins that function in DNA replication. DNA repair. cell cycle progression and chromatin assembly. It has been shown that differential posttranslational modifications of PCNA by ubiquitin or SUMO play a pivotal role in controlling the choice of pathway for rescuing stalled replication forks. Here, we explored the roles of Mgs1 and PCNA in replication fork rescue. We provide evidence that Mgs1 physically associates with PCNA and that Mgs1 helps suppress the RAD6 DNA damage tolerance pathway in the absence of exogenous DNA damage. We also show that PCNA sumoylation inhibits the growth of mgs1 rad18 double mutants, in which PCNA sumoylation and the Srs2 DNA helicase coordinately prevent RAD52-dependent homologous recombination. The proposed roles for Mgs1. Srs2. and modified PCNA during replication arrest highlight the importance of modulating the RAD6 and RAD52 pathways to avoid genome instability.