Genome digestion is a dispensable consequence of physiological cell death mediated by cytotoxic T lymphocytes.
Genome digestion is a dispensable consequence of physiological cell death mediated by cytotoxic T lymphocytes.
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基因组消化是细胞毒性 T 淋巴细胞介导的生理细胞死亡的一个可有可无的结果。
DOI：
10.1128/mcb.12.7.3060-3069.1992
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发表时间：
1992
影响因子：
5.3
通讯作者：
Meyers,J
中科院分区：
文献类型：
--
作者：
Ucker,DS;Obermiller,PS;Eckhart,W;Apgar,JR;Berger,NA;Meyers,J
我们检测了病毒转化的小鼠成纤维细胞克隆作为细胞毒性Τ淋巴细胞（CTL）触发的裂解和基因组消化的靶标。引人注目的是，虽然所有克隆在裂解能力上基本相同，但一个克隆SV3T3-B2.1未能表现出与CTL攻击相关的基因组消化。生理细胞死亡过程的其他方面，包括粘附丧失和核膜破裂（纤层蛋白磷酸化和溶解），在该克隆中没有改变。与ctl诱导的细胞死亡相关的基因组消化缺失与该克隆细胞核中缺乏内脱氧核糖核酸酶活性相关。受影响活性的表征确定了一种钙依赖的，大约40 kDa的dna酶i样内切酶，通常存在于所有细胞核中，作为负责与ctl介导的细胞死亡相关的基因组消化的酶。这些观察结果表明，无论是基因组消化本身还是其后果（如多聚腺苷核糖聚合酶的激活）都不是触发细胞自杀过程导致的细胞死亡所必需的。
We examined virally transformed murine fibroblast clones as targets for cytotoxic Τ lymphocyte (CTL)-triggered lysis and genome digestion. Strikingly, while all clones were essentially equivalent in the ability to be lysed, one clone, SV3T3-B2.1, failed to exhibit genome digestion associated with CTL attack. Other aspects of the physiological cell death process, including loss of adhesion and nuclear envelope breakdown (lamin phosphorylation and solubilization), were not altered in this clone. The absence of genome digestion associated with CTL-induced cell death correlated with the absence of endodeoxyribonuclease activity in the nuclei of that clone. Characterization of the activity affected identifies a calcium-dependent, DNase I-like endonuclease of approximately 40 kDa, normally present constitutively in all cell nuclei, as the enzyme responsible for genome digestion associated with CTL-mediated cell death. These observations indicate that neither genome digestion per se nor its consequences [such as activation of poly(ADP-ribose) polymerase] are essential for cell death resulting from the triggering of this cell suicide process.