Targeting Anion Exchange of Osteoclast, a New Strategy for Preventing Wear Particles Induced- Osteolysis.
Targeting Anion Exchange of Osteoclast, a New Strategy for Preventing Wear Particles Induced- Osteolysis.
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DOI：
10.3389/fphar.2018.01291
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发表时间：
2018
影响因子：
5.6
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Wu C;Liu X;Sun R;Qin Y;Liu Z;Yang S;Tang T;Zhu Z;Yu D;Liu F
关节置换术是治疗严重关节疾病的必要手段。然而，假体失败仍然是一个重要的临床问题，由磨损颗粒诱导破骨细胞骨吸收引起的假体周围骨溶解（PO）是失败的主要原因。活化T细胞的核因子c1 （NFATc1）似乎在磨损颗粒诱导的破骨细胞形成中发挥重要作用，在破骨细胞形成过程中，碳酸氢盐/氯化物交换剂、溶质载体家族4、阴离子交换剂成员2 （SLC4A2）以NFATc1依赖的方式上调。SLC4A2介导的破骨细胞阴离子交换可通过调节pHi影响骨吸收活性。本研究探讨了SLC4A2在磨损颗粒诱导的破骨细胞分化和体外功能中的作用和机制。使用阴离子交换抑制剂4,4 ' -二异硫氰酸-2,2 ' -二苯乙烯二磺酸（DIDS）在体内抑制磨损颗粒诱导的PO。壳聚糖微球控释DIDS可增强对PO的治疗效果。因此，破骨细胞SLC4A2介导的阴离子交换可能是治疗人工关节无菌性松动的潜在治疗靶点。
Joint replacement is essential for the treatment of serious joint disease. However, prosthetic failure remains an important clinical issue, with periprosthesis osteolysis (PO), caused by osteoclastic bone resorption induced by wear particles, being the leading cause of failure. Nuclear factor of activated T cells c1 (NFATc1) appears to play an important role in wear particle-induced osteoclastogenesis, with bicarbonate/chloride exchanger, solute carrier family 4, anion exchanger, member 2, (SLC4A2) being upregulated during osteoclastogenesis in an NFATc1-dependent manner. Anion exchange mediated by SLC4A2 in osteoclasts could affect the bone resorption activity by regulating pHi. This study investigated the role and mechanism of SLC4A2 in wear particle-induced osteoclast differentiation and function in vitro. The use of 4, 4′-diisothiocyano-2,2′-stilbenedisulfonic acid (DIDS), an anion exchange inhibitor, suppressed wear particle-induced PO in vivo. Furthermore, controlled release of DIDS from chitosan microspheres can strengthen the PO therapy effect. Therefore, anion exchange mediated by osteoclastic SLC4A2 may be a potential therapeutic target for the treatment of aseptic loosening of artificial joints.
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影响因子：
2.8
作者：
Baumann, B;Seufert, J;Rader, CP
通讯作者：
Rader, CP
DOI：
10.1073/pnas.1206392110
发表时间：
2013-02-05
影响因子：
11.1
作者：
Coury, Fabienne;Zenger, Serhan;Aliprantis, Antonios O.
通讯作者：
Aliprantis, Antonios O.
影响因子：
3.4
作者：
Hua, SM;Inesi, G
通讯作者：
Inesi, G
新型 V-ATP 酶抑制剂水利苯卤酰胺通过抑制破骨细胞骨吸收来预防磨损颗粒引起的骨溶解
影响因子：
3.7
作者：
Qin A;Cheng TS;Lin Z;Cao L;Chim SM;Pavlos NJ;Xu J;Zheng MH;Dai KR
通讯作者：
Dai KR
影响因子：
8.2
作者：
Liu, Feng-xiang;Wu, Chuan-long;Tang, Ting-ting
通讯作者：
Tang, Ting-ting