Determination of peimine and peiminine in Bulbus Fritillariae Thunbergii by capillary electrophoresis by indirect UV detection using N-(1-naphthyl)ethylenediamine dihydrochloride as probe
Determination of peimine and peiminine in Bulbus Fritillariae Thunbergii by capillary electrophoresis by indirect UV detection using N-(1-naphthyl)ethylenediamine dihydrochloride as probe
复制标题
N-(1-萘基)乙二胺二盐酸盐为探针毛细管电泳间接紫外检测法测定贝母中贝母碱和贝母碱的含量
DOI：
10.1002/elps.201200021
复制
发表时间：
2012-07-01
期刊：
影响因子：
2.9
通讯作者：
Jia, Zhimin
中科院分区：
文献类型：
--
作者：
Liu, Lihong;You, Wenwei;Jia, Zhimin
建立了一种简便、廉价的毛细管电泳法测定贝母素甲和贝母素乙的含量。由于贝母素甲和贝母素乙没有紫外发色团，因此选择了以N-（1-萘基）乙二胺二盐酸盐（NED）为紫外吸收探针的间接紫外检测法。有人认为，NED，发色团离子，可能会形成氢键对与分析物，导致分离选择性的显着变化。此外，分析物和探针之间的疏水相互作用在实现两种分析物之间的分辨率方面也起着至关重要的作用。使用由66% MeOHACN（1：1，v/v）、含15 mM NaH 2 PO 4、2.5 mM NED、4 mM H3 PO 4的34%水性缓冲液组成的背景电解质进行分析。使用MeOHACN混合物作为有机修饰剂，不仅可以减少NED在毛细管壁上的吸附，而且可以降低基线噪声和漂移。该方法提供的线性响应范围为5至200 μ g/mL。贝母素甲和贝母素甲的检出限分别为3.9和4.1 μ g/mL。峰面积的相对标准偏差（RSD）分别为3.4%和4.1%，峰高的RSD分别为4.0%和4.4%，峰移行时间的RSD分别为0.29%和0.30%。回归方程显示各分析物的峰面积与浓度之间呈线性关系（r = 0.99950.9996）。该方法已成功地应用于浙贝母氯仿提取物中贝母素甲和贝母素乙的定量分析。
A simple and inexpensive CE method was developed for the determination of peimine and peiminine. Because of the lack of an UV chromophore of peimine and peiminine, the detection method chosen was indirect UV detection, with N-(1-naphthyl)ethylenediamine dihydrochloride (NED) as the UV absorbing probe. It was thought that NED, a chromophoric ion, may form hydrogen bonding pairs with the analytes to cause significant changes in separation selectivity. Additionally, the hydrophobic interactions between analytes and the probe also play a crucial role in achieving a resolution between the two analytes. The analyses were carried out with a background electrolyte composed of 66% MeOHACN (1:1, v/v), 34% aqueous buffer containing 15 mM NaH2PO4, 2.5 mM NED, 4 mM H3PO4. MeOHACN mixtures used as organic modifiers can not only reduce the adsorption of NED to the capillary wall, but also decrease the baseline noise and drift. The method provided a linear response ranging from 5 to 200 mu g/mL. The limits of detection (LODs) for peimine and peiminine were 3.9 and 4.1 mu g/mL, respectively. The repeatabilities (n = 3) reached relative standard deviation values (RSDs) of 3.4 and 4.1% for the peak areas, 4.0 and 4.4% for the peak heights, and 0.29 and 0.30% for the migration time of peimine and peiminine, respectively. Regression equations revealed linear relationships (r = 0.99950.9996) between the peak area of each analyte and the concentration. The method developed was successfully applied to quantify peimine and peiminine in chloroform extracts of the ground Bulbus Fritillariae Thunbergii.