Human embryonic stem cell-derived neural precursor transplants attenuate apomorphine-induced rotational behavior in rats with unilateral quinolinic acid lesions
Human embryonic stem cell-derived neural precursor transplants attenuate apomorphine-induced rotational behavior in rats with unilateral quinolinic acid lesions
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DOI：
10.1016/j.neulet.2007.05.066
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发表时间：
2007-08-09
影响因子：
2.5
通讯作者：
Kim, Manho
中科院分区：
文献类型：
--
作者：
Song, Jihwan;Lee, Soon-Tae;Kim, Manho
为了检测人胚胎干细胞（human embryonic stem cell，hESC）源性神经前体细胞在亨廷顿病（Huntington 'sdisease，HD）实验模型中的疗效，我们通过与PA6基质细胞共培养将hESC分化为nestin阳性的神经前体细胞，随后将其移植到喹啉酸（quinolinic acid，QA）诱导的HD模型的纹状体中。在阿扑吗啡诱导的旋转试验中，移植动物在移植后3周表现出行为恢复。移植的hESC衍生的神经前体在皮质和纹状体中均被发现。它们还表现出一些神经元分化的证据。检查时未发现肿瘤。这些结果强烈表明，hESC衍生的神经前体可以导致HD临床前模型中的行为恢复以及神经元分化。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
To test the efficacy of human embryonic stem cell (hESC)-derived neural precursors in an experimental model of Huntington's disease (HD), we differentiated hESC into nestin-positive neural precursors by co-culturing with PA6 stromal cells, and subsequently transplanted them into the striatum of quinolinic acid (QA)-induced HD model. The transplanted animals exhibited a behavioral recovery in the apomorphine-induced rotation test for 3 weeks after transplantation. The transplanted hESC-derived neural precursors were found in both cortex and striatum. They also exhibited some evidence of neuronal differentiation. At the time of examination, no tumor was detected. These results strongly suggest that hESC-derived neural precursors can lead to a behavioral recovery, as well as neuronal differentiation, in the pre-clinical model of HD. (C) 2007 Elsevier Ireland Ltd. All rights reserved.