Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years
Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years
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DOI：
10.1016/j.meegid.2015.04.019
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发表时间：
2015-07-01
影响因子：
3.2
通讯作者：
Tardy, Florence
中科院分区：
文献类型：
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作者：
Becker, Claire A. M.;Thibault, Francois M.;Tardy, Florence
牛支原体（M.）牛支原体最近已成为世界范围内牛呼吸道疾病的主要病原体，主要由于其高发病率和死亡率以及低生长速度而导致巨大的经济损失。据报道，牛支原体感染在不同动物、畜群、地区或国家之间的传播通常与动物的流动有关。然而，尽管最近取得了相当大的努力，但尚未有通用的分型方法可用于在国际范围内追踪牛分枝杆菌分离株的传播。此外，在法国，自20世纪90年代初以来一直没有对牛分枝杆菌分离株的总体种群多样性进行评估。这项研究就是为了填补这些空白。使用两种分子分型方法评估了过去35年来在法国收集的60株分离株的基因型多样性，这些方法既解决了分离株之间的长期流行病学关系（多位点序列分型，MLST），也解决了遗传微变异（多位点VNTR分析，MLVA）。利用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对分离菌株的主要蛋白谱进行了表型多样性分析。所有提出的亚型分型方法均经过优化，并在法国牛乳杆菌种群中得到相同的模式，该种群由两个集群组成，第一个集群包括2000年以前收集的分离株，第二个集群包括2000年以后收集的分离株。最近的菌株进一步显示出比以前的菌株更加同质，这与单一克隆在全国范围内的传播是一致的。由于这种传播伴随着多重耐药牛分枝杆菌分离株的出现，因此讨论了几种假设来解释法国牛分枝杆菌分离株的同质性，尽管牛分枝杆菌物种完全具备产生多样性的能力。(C) 2015 Elsevier B.V.版权所有
Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity. (C) 2015 Elsevier B.V. All rights reserved.