CELL DETACHMENT MEDIATED BY HYALURONIC-ACID
CELL DETACHMENT MEDIATED BY HYALURONIC-ACID
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DOI：
10.1016/0014-4827(82)90009-x
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
VECCHIA, P
中科院分区：
文献类型：
--
作者：
ABATANGELO, G;CORTIVO, R;VECCHIA, P
将外源性透明质酸（HA）加入到[小鼠成纤维细胞] 3T3 BALB细胞的融合单层中，可促进细胞脱离，轻轻移液可增强细胞脱离。当HA与细胞接种物一起添加到细胞培养物中时，细胞能够生长并形成汇合的单层，但细胞密度以浓度依赖性方式低于对照培养物。这种差异似乎是由于HA在融合附近的细胞上促进分离的容易性。仅在接近汇合时，含有HA的培养板中的脱离细胞的量大于对照。通过用不同的分离剂去除细胞来制备含有由汇合的3T3 BALB细胞留下的基底附着材料（SAM）的培养皿。将含有SAM的培养皿在HA存在下孵育24小时，洗涤后用于细胞培养。在这种HA处理的培养皿上生长的细胞在汇合时显示出非常低的密度，并且在某些情况下无法形成汇合的单层。当用链霉菌透明质酸酶处理含SAM的培养皿时，HA的作用被消除，细胞可以正常生长。在硫酸软骨素中，只有硫酸软骨素C显示出与HA相同的效果，而A和B无效。
Exogenous hyaluronic acid (HA) added to a confluent monolayer of [mouse fibroblast] 3T3 BALB cells facilitates cell detachment which can be enhanced by gently pipetting. When HA is added to a cell culture with the cell inoculum, the cells are able to grow and form a confluent monolayer, but the cellular density is lower than in the control cultures, in a concentration-dependent way. This difference seems due to the ease of detachment promoted by HA on the cells near confluency. Only near confluency is the amount of the detached cells greater in the culture plates containing HA than in controls. Culture dishes containing substrate-attached material (SAM) left behind by the confluent 3T3 BALB cells were prepared by removing the cells with different detaching agents. The SAM-containing dishes were incubated in the presence of HA for 24 h and, after washing, were used for cell cultures. The cells grown on such HA-treated dishes show a very low density at confluency and in some cases are prevented from forming a confluent monolayer. When the SAM-containing dishes are treated with Streptomyces hyaluronidase, the effect of HA is abolished and the cells can grow normally. Among the chondroitin sulfates, only chondroitin sulfate C shows the same effects as HA, whereas A and B are ineffective.