Serum N-glycan profiling can predict biopsy-proven graft rejection after living kidney transplantation.
Serum N-glycan profiling can predict biopsy-proven graft rejection after living kidney transplantation.
复制标题
血清 N-聚糖分析可以预测活体肾移植后经活检证实的移植物排斥反应。
DOI：
10.1007/s10157-019-01820-8
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发表时间：
2020
期刊：
影响因子：
2.3
通讯作者：
Ohyama C.
中科院分区：
文献类型：
--
作者：
Soma O;Hatakeyama S;Yoneyama T;Saito M;Sasaki H;Tobisawa Y;Noro D;Suzuki Y;Tanaka M;Nishimura SI;Harada H;Ishida H;Tanabe K;Satoh S;Ohyama C.
目的探讨血清N-葡聚糖谱能否作为活体供肾移植(KT)后排斥反应的诊断指标。方法对5个医学中心174例活体肾移植受者进行回顾性研究，采用糖印迹结合质谱仪分析患者术后血清N-葡聚糖水平。我们根据年龄、性别、免疫危险因素和KT后d1和d7血清N-糖蛋白水平的组合制定了一个综合评分来预测移植物排斥反应。无排斥反应存活率以积分之和(d1、 + 、d7)分层，采用Kaplan-Meier曲线。组)，122例受者未出现移植排斥反应(排斥阴性。组)。拒绝-POS组的总分显著高于正常组。与拒绝-阴性组相比，差异有统计学意义。一群人。KT后d1、d7积分的曲线下面积(AUC)分别为0.84和0.84。积分总和(D1、 + 、D7) ≥ 0.50判定移植肾排斥反应的敏感度为81%，特异度为80%，AUC值为0.87。积分总和较高的受者(D1、 + 、D7、 ≥ 、0.5)无排斥存活时间明显短于评分较低的受者。结论评价血清N-糖基化水平可识别易发生排斥的受者。
BackgroundTo evaluate whether serumN-glycan profile can be used as a diagnostic marker of graft rejection after living-donor kidney transplants (KT).MethodsWe retrospectively examined 174 KT recipients at five medical centers.N-Glycan levels were analyzed in postoperative serum samples using glycoblotting combined with mass spectrometry. We developed an integrated score to predict graft rejection based on a combination of age, gender, immunological risk factors, and serumN-glycan levels at post-KT day D1 and D7. Rejection-free survival rates stratified by the sum of integrated scores (D1 + D7) were evaluated using Kaplan–Meier curves.ResultsOf 174, 52 showed graft rejection (Rejection-pos. group) and 122 recipients did not show graft rejection (Rejection-neg. group). The integrated scores were significantly higher in the Rejection-pos. group than those of the Rejection-neg. group. Area-under-curve (AUC) value of integrated scores at post-KT D1, and D7 were 0.84 and 0.84, respectively. The sum of integrated scores (D1 + D7) ≥ 0.50 identified graft rejection with 81% sensitivity and 80% specificity; with an AUC value of 0.87. Recipients with higher sum of integrated scores (D1 + D7 ≥ 0.5) had significantly shorter rejection-free survival than those with lower scores.ConclusionEvaluation of serumN-glycosylation profiles can identify recipients who are prone to rejection.