DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase alpha to inhibition by butylphenyl-deoxyguanosine triphosphate.
DNA replication and UV-induced DNA repair synthesis in human fibroblasts are much less sensitive than DNA polymerase alpha to inhibition by butylphenyl-deoxyguanosine triphosphate.
复制标题
人类成纤维细胞中的 DNA 复制和紫外线诱导的 DNA 修复合成对丁基苯基脱氧鸟苷三磷酸的抑制作用远不如 DNA 聚合酶 α 敏感。
作者：
S. Dresler;M. Frattini
在哺乳动物细胞中，已知由高剂量紫外线辐射诱导的半保守 DNA 复制和 DNA 修复补片合成都会被 aphidicolin 抑制，表明 aphidicolin 敏感 DNA 聚合酶 α 和/或 δ 之一或两者参与这些过程。在本文中，N2-(对正丁基苯基)-2'-脱氧鸟苷-5'-三磷酸是聚合酶α的强抑制剂和聚合酶δ的弱抑制剂，用于进一步表征参与这两种形式的核DNA合成的DNA聚合酶。在渗透性人类成纤维细胞中，DNA 复制和紫外线诱导的 DNA 修复合成对抑制剂的抵抗力比 DNA 聚合酶 α 分别高约 500 和 3000 倍。这些发现与 DNA 聚合酶 δ 在这些过程中的参与最为一致。
In mammalian cells, both semiconservative DNA replication and the DNA repair patch synthesis induced by high doses of ultraviolet radiation are known to be inhibited by aphidicolin, indicating the involvement in these processes of one or both of the aphidicolin-sensitive DNA polymerases, alpha and/or delta. In this paper, N2-(p-n-butylphenyl)-2'-deoxyguanosine-5'-triphosphate, a strong inhibitor of polymerase alpha and a weak inhibitor of polymerase delta, is used to further characterize the DNA polymerase(s) involved in these two forms of nuclear DNA synthesis. In permeable human fibroblasts, DNA replication and ultraviolet-induced DNA repair synthesis are more resistant to the inhibitor than DNA polymerase alpha by factors of approximately 500 and 3000, respectively. These findings are most consistent with the involvement of DNA polymerase delta in these processes.