Rapid identification of staphylococci from prosthetic joint infections using MALDI-TOF mass-spectrometry
Rapid identification of staphylococci from prosthetic joint infections using MALDI-TOF mass-spectrometry
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DOI：
10.1177/039139881003300902
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发表时间：
2010-09-01
影响因子：
1.7
通讯作者：
Mack, Dietrich
中科院分区：
文献类型：
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作者：
Harris, Llinos G.;El-Bouri, Khalid;Mack, Dietrich
与植入医疗器械相关的医院获得性感染最常由葡萄球菌引起。目前的物种鉴定方法缓慢、昂贵，有时还不可靠。我们评估了Bruker Daltonics Microflex MALDI- tof /MS与MALDI Biotyper软件结合鉴定假体关节感染中158株特征葡萄球菌的能力，包括36株金黄色葡萄球菌、100株表皮葡萄球菌、10株头型葡萄球菌、8株卢敦葡萄球菌、2株warneri葡萄球菌和2株溶血葡萄球菌，采用Bruker Daltonics推荐的提取方法。MALDI Biotyper软件对所有分离株的物种鉴定是正确的，表明金黄色葡萄球菌和凝固酶阴性葡萄球菌之间的区分是可靠的。应用MALDI Biotyper软件的推荐标准，所有158株分离株的得分均为>= 2.0，这意味着所有分离株的属和种鉴定都是安全的。34/36的金黄色葡萄球菌、36/100的表皮葡萄球菌、5/10的头葡萄球菌、6/8的卢顿葡萄球菌、2/2的溶血葡萄球菌、0/2的瓦尔纳利葡萄球菌的评分为>= 2.3，表明极有可能是物种鉴定。表皮葡萄球菌25/100的分值接近2.3。看来，数据库中其他临床相关的葡萄球菌分离株可能有助于在分数上进行鉴定，这意味着极有可能的物种鉴定。MALDI Biotyper软件在一个物种群内识别克隆相关菌株（即亚分型）的能力进行了研究，并显示出巨大的潜力。综上所述，MALDI- tof /MS MALDI生物分型系统为临床分离的假体关节感染提供了一种快速、可靠的物种鉴定方法，并具有分型的潜力。
Hospital-acquired infections associated with implanted medical devices are most commonly caused by staphylococci. Current methods of species identification are slow, costly, and sometimes unreliable. We evaluated the ability of a Bruker Daltonics Microflex MALDI-TOF/MS in conjunction with MALDI Biotyper software to identify 158 characterized staphylococcal isolates from prosthetic joint infections, including 36 Staphylococcus aureus, 100 Staphylococcus epidermidis, 10 Staphylococcus capitis, 8 Staphylococcus lugdunensis, 2 Staphylococcus warneri, and 2 Staphylococcus haemolyticus isolates using the extraction method recommended by Bruker Daltonics. The suggested species identification by the MALDI Biotyper software was correct for all isolates, indicating reliable differentiation between S. aureus and coagulase-negative staphylococci. Applying the recommended criteria of the MALDI Biotyper software all 158 isolates gave scores >= 2.0, implying secure genus and probable species identification for all isolates. 34/36 S. aureus, 36/100 S. epidermidis, 5/10 S. capitis, 6/8 S. lugdunensis, 2/2 S. haemolyticus, 0/2 S. warneri displayed scores >= 2.3 implying highly probable species identification. For S. epidermidis 25/100 additional isolates had a score close to 2.3. It appears that additional clinically relevant staphylococcal isolates in the data base might aid in identification at scores implying highly probable species identification. The ability of the MALDI Biotyper software to recognize clonally-related strains within a species group (i.e. sub-typing) was investigated, and showed great potential. In conclusion, the MALDI-TOF/MS MALDI Biotyper system provides a promising rapid and reliable method of identifying clinical isolates from prosthetic joint infections to the species level, and has potential for sub-typing.