AGING RAT NEUROMUSCULAR-JUNCTIONS - A MORPHOMETRIC STUDY OF CHOLINESTERASE-STAINED WHOLE MOUNTS AND ULTRASTRUCTURE
AGING RAT NEUROMUSCULAR-JUNCTIONS - A MORPHOMETRIC STUDY OF CHOLINESTERASE-STAINED WHOLE MOUNTS AND ULTRASTRUCTURE
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DOI：
10.1002/mus.880100303
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发表时间：
1987-03-01
期刊：
影响因子：
3.4
通讯作者：
LAFONTAINE, DM
中科院分区：
文献类型：
--
作者：
CARDASIS, CA;LAFONTAINE, DM
先前对成年大鼠比目鱼肌神经肌肉接头（NMJ）的形态学研究表明，其超微结构随着年龄的增长而变化，这是突触前和突触后元件动态、持续重塑的结果。随着衰老，轴突萌芽和退缩之间的平衡似乎转向有利于去神经支配。在这里，我们通过电子显微镜复合材料和胆碱酯酶 (ChE) 染色的整体中所见的 NMJ 结构的量化和形态测量，检查了大鼠成年期间（5-111 周）重塑过程的程度和方向。我们发现轴突萌芽和退缩对整个 NMJ 区域的净效应随年龄的变化而变化：5-12 周时，轴突生长和成熟迅速； 12-48周时生长减慢，48-82周时方向逐渐转变，导致单个NMJ的突触接触逐渐丧失；最后，在第 82 至 111 周时，整个 NMJ 丧失，但其他神经仍继续恢复。隔膜NMJ还展现出持续的结构重塑；然而，在 82-111 周之间，ChE 染色模式的许多变化突然出现。
Previous morphological studies of the adult rat soleus neuromuscular junction (NMJ) indicate that its ultrastructure changes with age, a consequence of a dynamic, ongoing remodeling of pre- and postsynaptic elements. With aging, the balance between axonal sprouting and withdrawal appears to shift in favor of denervation. Here we have examined the extent and direction of the remodeling process during the adult life of the rat (5-111 weeks) by quantification and morphometry of NMJ structure as seen in electron microscopic composites and in cholinesterase (ChE) stained whole mounts. We have found that the net effect of axonal sprouting and withdrawal on the entire NMJ region varies with age: at 5-12 weeks there is rapid growth and maturation; at 12-48 weeks slower growth occurs, and at 48-82 weeks there is a gradual shift in direction to result in progressive loss of synaptic contact in individual NMJs; finally, at 82-111 weeks, there is loss of entire NMJs with some continued reinnervation at others. The diaphragm NMJ also exhiubits continuous structural remodeling; however, between 82-111 weeks many changes in the ChE staining pattern appear abruptly.