Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer
Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer
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DOI：
10.1021/ol0623926
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发表时间：
2006-12-21
期刊：
影响因子：
5.2
通讯作者：
Nagano, Tetsuo
中科院分区：
文献类型：
--
作者：
Mineno, Tomoko;Ueno, Tasuku;Nagano, Tetsuo
羧基荧光素被广泛用作荧光标记试剂，但我们最近发现其酯化后发射强度明显降低。基于我们的假设，荧光降低是由于供体激发的光诱导电子转移（d- PeT）过程，我们开发了新的羧基荧光素衍生物，其中d- PeT过程受到阻碍，并且在酯化过程中发射强度不会降低。这些新型染料衍生物显示出高量子产率，有望用作标记剂。
Carboxyfluoresceins are widely utilized as fluorescence labeling reagents, but we recently found that their emission intensity is markedly decreased after esterification. On the basis of our hypothesis that the fluorescence decrease is due to a donor- excited photoinduced electron transfer (d- PeT) process, we have developed novel carboxyfluorescein derivatives in which the d- PeT process is hampered, and the emission intensity is not decreased upon esterification. These novel dye derivatives display high quantum yields and are expected to be useful as labeling agents.