Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone
Production of 26-deoxymonensins A and B by Streptomyces cinnamonensis in the presence of Metyrapone
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DOI：
10.1128/aem.60.5.1561-1564.1994
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发表时间：
1994-05
影响因子：
4.4
通讯作者：
S. Pospíšil;P. Sedmera;V. Havlíček;J. Tax
中科院分区：
文献类型：
--
作者：
S. Pospíšil;P. Sedmera;V. Havlíček;J. Tax
甲吡酮，一种有效的细胞色素P-450抑制剂，以9 mM加入到肉桂链霉菌的浸没培养物中，引起总莫能菌素生物合成和新代谢产物26-脱氧莫能菌素A和B的共同产生的部分抑制。后者被分离为其25-O-甲基衍生物。甲吡酮同时还原为甲吡波尔。所有这些化合物都通过核磁共振光谱和质谱鉴定。
Metyrapone, a potent cytochrome P-450 inhibitor, added at 9 mM to a submerged culture of Streptomyces cinnamonensis caused partial inhibition of total monensin biosynthesis and coproduction of new metabolites, 26-deoxymonensins A and B. The latter was isolated as its 25-O-methyl derivative. Metyrapone was simultaneously reduced to metyrapol. All of these compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry.