Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus.
Complete sequences of the intergenic and mRNA start signals in the Sendai virus genome: homologies with the genome of vesicular stomatitis virus.
复制标题
仙台病毒基因组中基因间和 mRNA 起始信号的完整序列：与水泡性口炎病毒基因组的同源性。
DOI：
--
复制
发表时间：
1984
影响因子：
14.9
通讯作者：
D. Kingsbury
中科院分区：
文献类型：
--
作者：
K. Gupta;D. Kingsbury
副粘病毒仙台病毒基因组的所有基因间序列和转录起始序列均已确定。基因间序列3'- gaa和mRNA起始信号3‘- UCCCANUUUC之间的边界是通过对特定病毒mRNA分子的5’端进行测序确定的。5个基因间三核苷酸中有1个不同于其他的，由3'- GGG组成，并且在两个mRNA起始信号中观察到单碱基取代。仙台病毒基因间序列与水疱性口炎病毒（VSV）的类似序列（3′-GA）相似，VSV是另一种负链RNA病毒——横纹肌病毒家族的成员，但两种病毒的mRNA起始信号之间没有序列同源性。然而，这些mRNA起始信号以相同的方式组织，有10个碱基长，具有两个一致区域，由一个（仙台病毒）或两个（VSV）可变的内部核苷酸划分。这些发现进一步证明了负链RNA病毒的两个家族起源于一个共同的祖先，并且在它们的进化过程中保留了转录调控的原型机制。
All of the consensus intergenic and transcription initiation sequences of the genome of Sendai virus, a paramyxovirus, have been determined. The boundary between the intergenic sequence, 3'-GAA, and the mRNA start signal, 3'- UCCCANUUUC , was identified by sequencing the 5' termini of specific viral mRNA molecules. One of the five intergenic trinucleotides differed from the rest, consisting of 3'- GGG , and single base substitutions were observed in two of the mRNA start signals. The Sendai virus intergenic sequence was similar to the analogous sequence (3'-GA) of vesicular stomatitis virus (VSV), a member of another family of negative-strand RNA viruses, the rhabdoviruses , but there was no sequence homology between the mRNA start signals of the two viruses. Nevertheless, these mRNA start signals were organized in the same way, being ten bases long and possessing two consensus regions, divided by one (Sendai virus) or two (VSV) variable internal nucleotides. These findings extend the evidence that both families of negative-strand RNA viruses descended from a common ancestor and that an archetypal mechanism of transcriptional regulation has been conserved in their evolution.