Demonstration of a bio-microactuator powered by cultured cardiomyocytes coupled to hydrogel micropillars
Demonstration of a bio-microactuator powered by cultured cardiomyocytes coupled to hydrogel micropillars
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DOI：
10.1016/j.snb.2005.11.063
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发表时间：
2006-11-24
影响因子：
8.4
通讯作者：
Kitamori, Takehiko
中科院分区：
文献类型：
--
作者：
Morishima, Keisuke;Tanaka, Yo;Kitamori, Takehiko
虽然之前关于集成化学系统的基于电池的设备的工作主要集中在开发细胞的生化功能上，但我们展示了车载电池作为微执行器的直接利用，将化学能转换为机械能。利用心肌细胞驱动水凝胶微结构，论证了生物微致动器的概念。通过复制模塑方法制成的水凝胶微柱阵列，并针对细胞附着进行了修改，构成了用于心肌细胞的支撑支架。将原代新生大鼠心肌细胞直接培养到微柱上，成功地附着在微柱上。连接到细胞的微柱在没有任何生化触发的情况下有规律地、自发地跳动。在20℃时拍频约为0.1 Hz，观察到最强的柱状位移位移为6微米，根据微柱位移量估算培养心肌细胞的收缩力。观察到单个或少数附着在柱子上的心肌细胞的总自然收缩力超过8×10(-8)N。所展示的能力应该会使致动器和细胞杂交仪器的概念发生根本变化。(C)2005 Elsevier B.V.保留所有权利。
While previous works on cell-based devices for integrated chemical systems have focused on exploiting biochemical functions of cells, we demonstrated the direct utilization of on-board cells as microactuators converting chemical energy into mechanical energy. The concept of biomicroactuators was demonstrated by using cardiomyocytes to drive hydrogel microstructures. Arrays of hydrogel micropillars made by the replica molding method and modified for cell attachment comprised a supporting scaffold for cardiomyocytes. Primary neonatal rat cardiomyocytes were then cultured directly onto the micropillars, attaching to micropillars successfully. Micropillars coupled to cells beat regularly and spontaneously without any biochemical triggers. The beat frequency was about 0.1 Hz at 20 degrees C, and the pillar peak displacement beating most strongly in our observation was 6 mu m. The contractile force of the cultured cardiomyocytes was estimated by the displacement of the micropillar. Total natural contractile forces of single or a few cardiomyocytes attached to the pillar were observed to exceed 8 x 10(-8) N. The demonstrated capabilities should enable fundamental changes in the concept of actuators and cell-hybridized instrumentation. (c) 2005 Elsevier B.V. All rights reserved.