The effects of gradients of nerve growth factor immobilized PCLA scaffolds on neurite outgrowth in vitro and peripheral nerve regeneration in rats
The effects of gradients of nerve growth factor immobilized PCLA scaffolds on neurite outgrowth in vitro and peripheral nerve regeneration in rats
复制标题
神经生长因子固定PCLA支架梯度对大鼠体外神经突生长和周围神经再生的影响
DOI：
10.1016/j.biomaterials.2013.05.080
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发表时间：
2013-09-01
期刊：
影响因子：
14
通讯作者：
Quan, Daping
中科院分区：
文献类型：
--
作者：
Tang, Shuo;Zhu, Jixiang;Quan, Daping
将神经生长因子（NGF）浓度梯度导入导管内修复周围神经损伤，对神经再生和引导至关重要。本文结合NGF/丝素蛋白（SF）涂层的差异吸附，成功制备了梯度的NGF固定化膜（G-Ms）和纳米纤维神经导管（G-nNC）。通过定量比较背根神经节（DRG）神经突在G-MS或均匀的NGF固定膜（U-MS）上的生长，证实了NGF梯度的功效。值得注意的是，G-M组的神经突转动率为0.48 +/- 0.11，但U-M组接近于零。G-M组与U-M组相比，尽管平均NGF浓度相近，但G-M组中间的DRG神经突长度明显延长。此外，植入大鼠坐骨神经损伤14 mm间隙12周后，G-nNCs获得了令人满意的神经再生结果，与形态和功能的改善相关，这上级均匀的NGF固定的nNCs（U-nNCs）。坐骨神经功能指数（SFI），复合肌肉动作电位（CMAP），有髓神经纤维总数，髓鞘厚度与G-nNC和自体移植物相似，与G-nNC具有更高的轴突密度比自体移植物。我们的研究结果表明，在支架中引入NGF梯度在促进神经再生中的重要作用。(C)2013爱思唯尔有限公司保留所有权利。
Introducing concentration gradients of nerve growth factor (NGF) into conduits for repairing of peripheral nerve injury is crucial for nerve regeneration and guidance. Herein, combining differential adsorption of NGF/silk fibroin (SF) coating, the gradient of NGF-immobilized membranes (G-Ms) and nanofibrous nerve conduits (G-nNCs) were successfully fabricated. The efficacy of NGF gradients was confirmed by a quantitative comparison of dorsal root ganglia (DRG) neurite outgrowth on the G-Ms or uniform NGF-immobilized membranes (U-Ms). Significantly, the neurite turning ratio was 0.48 +/- 0.11 for G-M group, but it was close to zero for U-M group. The neurite length of DRGs in the middle of the G-Ms was significantly longer than that of U-M group, even though the average NGF concentration was approximated. Furthermore, 12 weeks after implantation in rats with a 14 mm gap of sciatic nerve injury, G-nNCs achieved satisfying outcomes of nerve regeneration associated with morphological and functional improvements, which was superior to that of the uniform NGF-immobilized nNCs (U-nNCs). Sciatic function index (SFI), compound muscle action potentials (CMAPs), total number of myelinated nerve fibers, thickness of myelin sheath were similar for the G-nNCs and autografts, with the G-nNCs having a higher density of axons than the autografts. Our results demonstrated the significant role of introducing NGF gradients into scaffolds in promoting nerve regeneration. (C) 2013 Elsevier Ltd. All rights reserved.