Protection against Fasciola hepatica in the intestine is highly correlated with eosinophil and immunoglobulin G1 responses against newly excysted juveniles
Protection against Fasciola hepatica in the intestine is highly correlated with eosinophil and immunoglobulin G1 responses against newly excysted juveniles
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肠道内对肝片形吸虫的保护与嗜酸性粒细胞和免疫球蛋白 G1 对新胞出的幼体的反应高度相关
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
B. Bokhout
中科院分区：
文献类型：
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作者：
F. V. van Milligen;J. Cornelissen;B. Bokhout
大鼠感染肝片吸虫，使用本实验室开发的体外肠环技术，每隔38周定期激发一次。对肠道组织和血清中观察到的抗肝片吸虫免疫反应的动力学进行了研究，并与保护相关。免疫组织化学方法检测肠环中嗜酸性粒细胞、免疫球蛋白(Ig)E阳性细胞和粘膜肥大细胞的频率，并确定新切除的幼鱼是否被抗体包裹或被嗜酸性粒细胞包围，或两者兼而有之。用酶联免疫吸附试验和放射免疫试验检测初检仔鱼的血清抗体反应。结果表明，保护性与肠道中嗜酸性粒细胞和IgE阳性细胞的频率高度相关，而与粘膜肥大细胞的频率仅中等相关。从表现出高水平保护的大鼠身上取出的新培育的幼鼠总是被免疫球蛋白抗体包裹，并被嗜酸性粒细胞包围。保护作用与血清中针对初检幼鱼的IgG1抗体效价高度相关，而与血清中IgA和IgE抗体效价的相关性较弱。由于保护性与肠道组织和血清中的IgG1以及肠道组织中的嗜酸性粒细胞高度相关，我们认为IgG1和嗜酸性粒细胞在保护大鼠抵抗肝片吸虫中起重要作用。
Rats were infected with Fasciola hepatica and challenged at regular intervals up to 38 weeks using an ex vivo gut loop, a technique developed in our laboratory. The kinetics of the observed immune responses against F. hepatica in gut tissue and serum were investigated and correlated to protection. Immunohistochemical methods were used to measure the frequency of eosinophils, immunoglobulin (Ig)E‐positive cells, and mucosal mast cells in the gut loop, and to determine whether the newly excysted juveniles were coated with IgG antibodies or surrounded by eosinophils, or both. Enzyme‐linked immunosorbent assays and a radioimmuno assay were used to measure serum antibody reactive with newly excysted juveniles. Results showed that protection was highly correlated with the frequency of eosinophils and IgE‐positive cells in the gut, but was only moderately correlated with the frequency of mucosal mast cells. Newly excysted juveniles taken from rats exhibiting high levels of protection were always coated with IgG antibodies and surrounded by eosinophils. Protection was highly correlated with titers of serum IgG1 antibodies directed against newly excysted juveniles, but was only weakly correlated with titers of serum IgA and IgE antibodies. Because protection was highly correlated with IgG1 in gut tissue and serum, and with eosinophils in gut tissue, we suggest that IgG1 and eosinophils are important in protecting rats against F. hepatica.