A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system.
A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system.
复制标题
使用 Cre-loxP 系统对靶基因进行 β 细胞特异性消融的小鼠模型。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
F. Brunicardi
中科院分区：
文献类型：
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作者：
M. K. Ray;S. P. Fagan;S. Moldovan;F. DeMayo;F. Brunicardi
大鼠胰岛素启动子 (RIP) 已被用来驱动 Cre 重组酶 (Cre) 在 β 细胞中的表达。在小鼠胰岛素瘤细胞系、NIT-1 和对照细胞系中进行瞬时转染。使用来自 RIP-Cre 转基因小鼠的胰腺和其他组织的总 RNA 进行 RT-PCR。此外，通过将 RIP-Cre 转基因小鼠与带有 beta-actin-loxP-CAT-loxP-lacZ 转基因的报告小鼠杂交，进一步分析了 RIP 的效率和特异性。在这些小鼠中，lacZ 仅在通过 Cre 介导的重组切除 floxed-CAT 基因后才表达。在这里，我们展示了双基因小鼠中 lacZ 的 β 细胞特异性表达的数据，作为针对 β 细胞特异性基因的小鼠模型中的概念证明。 RIP-Cre 转基因小鼠将被用作靶向切除 β 细胞特异性基因的潜在工具，以研究它们在胰岛细胞生理学中的作用。
The rat insulin promoter (RIP) has been used to drive the expression of Cre recombinase (Cre) specifically in beta cells. Transient transfection was performed in the mouse insulinoma cell line, NIT-1, and control cell lines. RT-PCR was performed using total RNA from pancreas and other tissues of RIP-Cre transgenic mice. In addition, the efficiency and specificity of RIP were further analyzed by cross breeding the RIP-Cre transgenic mice with reporter mice bearing a beta-actin-loxP-CAT-loxP-lacZ transgene. In these mice, lacZ is expressed only after excision of the floxed-CAT gene by Cre-mediated recombination. Here, we present the data for beta cell-specific expression of lacZ in bigenic mice, as proof of concept in a mouse model for targeting beta cell-specific gene(s). The RIP-Cre transgenic mice will be used as a potential tool for targeting the excision of beta cell-specific gene(s) to study their role in islet cell physiology.