Periglaucines A-D, anti-HBV and -HIV-1 alkaloids from Pericampylus glaucus
Periglaucines A-D, anti-HBV and -HIV-1 alkaloids from Pericampylus glaucus
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Periglaucines A-D、来自 Pericampylus glaucus 的抗 HBV 和 -HIV-1 生物碱
DOI：
10.1021/np070479
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发表时间：
2008-05-01
影响因子：
5.1
通讯作者：
Chen, Ji-Jun
中科院分区：
文献类型：
--
作者：
Yan, Meng-Hong;Cheng, Pi;Chen, Ji-Jun
从灰绿Pericampylus glaucus的地上部分分得4个新的hasubanane型生物碱，即Periglaucines A-D（1-4）和3个已知生物碱，即norruffscine（5），（-）-8-oxotetrahydropalmatine（6）和（-）-8-oxocanadine（7）。它们的结构经核磁共振谱和电子轰击质谱确证，其中围苷A（1）的结构经单晶X-射线衍射确证。生物碱1-4抑制Hep G2.2.15细胞中的B型肝炎病毒（HBV）表面抗原（HBsAg）分泌。（-）-8-氧代四氢巴马汀（6）具有较高的选择性（SI = 22.4），IC（50）值为0.14 mM。去甲番荔枝碱（5）和（-）-8-氧代四氢巴马汀（6）显示出对人类免疫缺陷病毒（HIV-1）的抑制活性，EC（50）在C8166细胞中的值分别为10.9和14.1 μ M（SI = 45.7和18.8）。
Four new hasubanane-type alkaloids, periglaucines A-D (1-4), and three known alkaloids, norruffscine (5), (-)-8-oxotetrahydropalmatine (6), and (-)-8-oxocanadine (7), were isolated from the aerial parts of Pericampylus glaucus. Their structures were elucidated on the basis of extensive NMR and EIMS data, and that of periglaucine A (1) was confirmed by single-crystal X-ray diffraction. Alkaloids 1-4 inhibited hepatitis B virus (HBV) surface antigen (HBsAg) secretion in Hep G2.2.15 cells. (-)-8-Oxotetrahydropalmatine (6) possessed a high selectivity index (SI = 22.4) for HBsAg secretion of the Hep G2.2.15 cell line with an IC(50) value of 0.14 mM. Norruffscine (5) and (-)-8-oxotetrahydropalmatine (6) exhibited inhibitory activity against human immunodeficiency virus (HIV-1) with EC(50) values of 10.9 and 14.1 mu M in C8166 cells (SI = 45.7 and 18.8), respectively.