Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression
Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression
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DOI：
10.1016/s0092-8674(00)80099-9
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发表时间：
1996-07-26
期刊：
影响因子：
64.5
通讯作者：
Hieter, P
中科院分区：
文献类型：
--
作者：
Connelly, C;Hieter, P
发芽酵母Skp1基因被认为是一个已知动粒蛋白突变体的剂量抑制因子，它编码CBF3的一个内在22.3 kDa亚基，CBF3是一个在体外与着丝粒DNA结合的多蛋白复合体。Skp1的温度敏感突变定义了两个不同的表型类别。Skp1-4突变体主要作为具有G2DNA含量和短有丝分裂纺锤体的大型萌发细胞停滞，这与动粒功能中的一个作用一致，然而，Skp1-3突变体主要作为具有G1DNA含量的多个萌发细胞停滞，这表明在G1/S阶段有额外的作用。对线虫、拟南线虫和智人的Skp1p同源物的鉴定表明，Skp1在进化上高度保守。因此，Skp1p代表着一种在真核生物进化过程中保守的内在动粒蛋白，并可能直接参与连接动粒功能与细胞周期调节机制。
The budding yeast SKP1 gene, identified as a dosage suppressor of a known kinetochore protein mutant, encodes an intrinsic 22.3 kDa subunit of CBF3, a multi-protein complex that binds centromere DNA in vitro. Temperature-sensitive mutations in SKP1 define two distinct phenotypic classes. skp1-4 mutants arrest predominantly as large budded cells with a G2 DNA content and short mitotic spindle, consistent with a role in kinetochore function, skp1-3 mutants, however, arrest predominantly as multiply budded cells with a G1 DNA content, suggesting an additional role during the G1/S phase. Identification of Skp1p homologs from C. elegans, A. thaliana, and H. sapiens indicates that SKP1 is evolutionarily highly conserved. Skp1p therefore represents an intrinsic kinetochore protein conserved throughout eukaryotic evolution and may be directly involved in linking kinetochore function with the cell cycle-regulatory machinery.