SUBSTRATE DEPENDENCE OF CELL-MIGRATION FROM EXPLANTED NEURAL TUBES INVITRO
SUBSTRATE DEPENDENCE OF CELL-MIGRATION FROM EXPLANTED NEURAL TUBES INVITRO
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DOI：
10.1007/bf00399515
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发表时间：
1976-01-01
影响因子：
3.6
通讯作者：
MAXWELL, GD
中科院分区：
文献类型：
--
作者：
MAXWELL, GD
将含有神经脊细胞的鸡胚神经管体外培养在组织培养塑料和胶原上。测定了两个参数，即细胞从神经管开始迁移的时间和细胞前沿离开神经管外植体的速度。在胶原蛋白上，细胞迁移持续在体外4-6h后开始，大约比细胞在组织培养塑料上迁移开始的时间早5h。通过最终分化为黑素细胞，确定迁移细胞为神经脊细胞的身份。消融实验表明，胶原蛋白还导致非神经脊来源的细胞提前迁移。细胞外物质可以改变细胞在形态发生过程中的迁移行为。
Embryonic chick neural tubes containing neural crest cells were cultured in vitro on tissue culture plastic and collagen. Two parameters, the time of onset of cell migration from the neural tube and the rate of movement of the cell front away from the neural tube explant, were determined. On collagen, cell migration consistently began after 4-6 h in vitro, about 5 h prior to the onset of cell migration on tissue culture plastic. The identity of the migrating cells as neural crest cells was established by their eventual differentiation into melanocytes. Ablation experiments revealed that collagen also caused the early onset of migration of cells not of neural crest origin. Extracellular materials may alter cell migratory behavior in morphogenesis.