Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis
Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis
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DOI：
10.1007/s11240-008-9401-1
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发表时间：
2008-08-01
影响因子：
3
通讯作者：
Castano, E.
中科院分区：
文献类型：
--
作者：
Arroyo-Herrera, A.;Gonzalez, A. Ku;Castano, E.
细胞分化依赖于形态发生所需的关键基因的正确和顺序表达。几个方面的控制是必需的，其中包括：染色质修饰，DNA甲基化，正确的量的特定的转录因子，适当的核排列，等等。在过去的几年中，同源框转录因子WUSCHEL（WUS）已被证明会导致去分化时，体细胞上表达，然后通过生产新的干细胞，可以导致体细胞胚胎发生或器官发生。我们发现WUS在咖啡植物中的表达可以诱导愈伤组织的形成以及体细胞胚产量增加400%。结果表明，转录因子WUS的转基因表达可用于增加异源系统中的体细胞胚胎发生。然而，一个关键的发展阶段和额外的激素要求的诱导胚胎发生所需的WUS在咖啡。
Cell differentiation depends on the proper and sequential expression of key genes required for morphogenesis. Several aspects of control are required for this which include: chromatin modifications, DNA methylation, correct amount of particular transcription factors, proper nuclear arrangement, etc. During the last few years the homeobox transcription factor WUSCHEL (WUS) has been shown to cause dedifferentiation when expressed on somatic cells followed by a production of new stem cells that can lead to somatic embryogenesis or organogenesis. We found that expression of WUS in coffee plants can induce calli formation as well as a 400% increase somatic embryo production. The results show that transgenic expression of the transcription factor WUS can be useful to increase somatic embryogenesis in heterologous systems. However, a critical developmental stage and additional hormonal requirements are required for the induction of embryogenesis by WUS in Coffea canephora.