Profiling of differentially expressed microRNAs in arrhythmogenic right ventricular cardiomyopathy.
Profiling of differentially expressed microRNAs in arrhythmogenic right ventricular cardiomyopathy.
复制标题
致心律失常性右心室心肌病中差异表达 microRNA 的分析
DOI：
10.1038/srep28101
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发表时间：
2016-06-16
影响因子：
4.6
通讯作者：
Wei Y
中科院分区：
文献类型：
--
作者：
Zhang H;Liu S;Dong T;Yang J;Xie Y;Wu Y;Kang K;Hu S;Gou D;Wei Y
致心律失常性右心室心肌病（ARVC）是一种以右心室心肌纤维脂肪替代为特征的原发性心肌病。目前，心肌microRNA已被报道在心血管病理生理学的病理生理学中发挥关键作用。迄今为止，ARVC 中 microRNA 的分析尚未被描述。在本研究中，我们应用S-Poly (T) Plus方法研究了24例ARVC患者心脏样本中microRNA的表达谱。对 1078 种人类 microRNA 的组织水平进行了评估，并与 24 名健康对照者的水平进行了比较。受试者工作特征曲线（ROC）下面积分析支持 21 种经过验证的 microRNA 是 ARVC 的 miRNA 特征，11 种 microRNA 在 ARVC 心脏组织中显着增加，10 种 microRNA 显着减少。经过功能富集分析，miR-21-5p和miR-135b与Wnt和Hippo通路相关，可能参与ARVC的分子病理生理学。总体而言，我们的数据表明心肌 microRNA 参与 ARVC 的病理生理学，miR-21-5p 和 miR-135b 与心肌脂肪和纤维化显着相关，这是 ARVC 的潜在疾病途径，可能可作为 ARVC 的治疗靶点。
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a kind of primary cardiomyopathy characterized by the fibro-fatty replacement of right ventricular myocardium. Currently, myocardial microRNAs have been reported to play critical role in the pathophysiology of cardiovascular pathophysiology. So far, the profiling of microRNAs in ARVC has not been described. In this study, we applied S-Poly (T) Plus method to investigate the expression profile of microRNAs in 24 ARVC patients heart samples. The tissue levels of 1078 human microRNAs were assessed and were compared with levels in a group of 24 healthy controls. Analysis of the area under the receiver operating characteristic curve (ROC) supported the 21 validated microRNAs to be miRNA signatures of ARVC, eleven microRNAs were significantly increased in ARVC heart tissues and ten microRNAs were significantly decreased. After functional enrichment analysis, miR-21-5p and miR-135b were correlated with Wnt and Hippo pathway, which might involve in the molecular pathophysiology of ARVC. Overall, our data suggested that myocardial microRNAs were involved in the pathophysiology of ARVC, miR-21-5p and miR-135b were significantly associated with both the myocardium adipose and fibrosis, which was a potential disease pathway for ARVC and might to be useful as therapeutic targets for ARVC.