OB-fold Families of Genome Guardians: A Universal Theme Constructed From the Small β-barrel Building Block.
OB-fold Families of Genome Guardians: A Universal Theme Constructed From the Small β-barrel Building Block.
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DOI：
10.3389/fmolb.2022.784451
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发表时间：
2022
影响因子：
5
通讯作者：
Bianco PR
中科院分区：
文献类型：
--
作者：
Bianco PR
基因组稳定性的维持需要多种蛋白质和蛋白质复合物的协调作用，这些蛋白质和蛋白质复合物统称为基因组监护人。在这个广泛定义的家族内，是含有寡核苷酸/寡糖结合折叠（OB-折叠）的蛋白质的子集。虽然OB-折叠与单链DNA的结合广泛相关，但这种观点不再是如何利用这些结构域的准确描述。相反，OB-折叠的核心被修饰并适应于促进与各种DNA底物（单链和双链）、磷脂和蛋白质的结合，以及使多亚基复合物具有催化功能。伴随着独特的寡聚化状态和四级结构的灵活性使OB-折叠基因组监护人能够通过原核生物和真核生物中无数复杂和动态的蛋白质-蛋白质、蛋白质-DNA和蛋白质-脂质相互作用来维持基因组的完整性。
The maintenance of genome stability requires the coordinated actions of multiple proteins and protein complexes, that are collectively known as genome guardians. Within this broadly defined family is a subset of proteins that contain oligonucleotide/oligosaccharide-binding folds (OB-fold). While OB-folds are widely associated with binding to single-stranded DNA this view is no longer an accurate depiction of how these domains are utilized. Instead, the core of the OB-fold is modified and adapted to facilitate binding to a variety of DNA substrates (both single- and double-stranded), phospholipids, and proteins, as well as enabling catalytic function to a multi-subunit complex. The flexibility accompanied by distinctive oligomerization states and quaternary structures enables OB-fold genome guardians to maintain the integrity of the genome via a myriad of complex and dynamic, protein-protein; protein-DNA, and protein-lipid interactions in both prokaryotes and eukaryotes.