Effect of a probiotic fermented milk on the thymus in Balb/c mice under non-severe protein-energy malnutrition
Effect of a probiotic fermented milk on the thymus in Balb/c mice under non-severe protein-energy malnutrition
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DOI：
10.1017/s0007114512005302
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发表时间：
2013-08-14
影响因子：
3.6
通讯作者：
Perdigon, Gabriela
中科院分区：
文献类型：
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作者：
Novotny Nunez, Ivanna;Maldonado Galdeano, Carolina;Perdigon, Gabriela
蛋白质能量营养不良(PEM)会导致免疫系统的严重损害，胸腺是受影响最严重的器官之一。在BALB/c小鼠非严重营养不良模型中，益生菌发酵乳(PFM)能恢复肠道屏障、组织学改变以及粘膜和系统免疫功能。本研究的目的是在相同的营养不良模型中，评估在再营养饮食中添加PFM对胸腺恢复的影响，分析营养不良引起的组织学和功能变化。小鼠营养不良，根据再营养期间摄入的膳食补充剂分为三组：牛奶、PFM或其无菌上清液(BFS)。将它们与营养良好和营养不良的小鼠进行比较。PFM是改善胸腺组织学、减少胸腺细胞凋亡、恢复CD4(+)/CD8(-)单一阳性胸腺细胞百分比的最有效的营养补充剂。营养不良对照组(MC)未成熟双阳性胸腺细胞增多。与接受其他膳食补充剂和MC的小鼠相比，服用PFM的小鼠胸腺中不同细胞因子的产生增加。给予BFS的小鼠的胸腺表现出与接受牛奶的小鼠相似的改善。在非重度PEM模型中，我们证明了整个PFM补充对胸腺的组织学和功能恢复的重要性。
Protein-energy malnutrition (PEM) causes a significant impairment of the immune system, the thymus being one of the most affected organs. It has been demonstrated that the administration of probiotic fermented milk (PFM) recovered the intestinal barrier, histological alterations and mucosal and systemic immune functions in a non-severe malnutrition model using BALB/c mice. The aim of the present study was to evaluate, in the same model of malnutrition, the effect of a PFM added to a re-nutrition diet on the recovery of the thymus, analysing histological and functional alterations caused by malnutrition. Mice were undernourished and divided into three groups according to the dietary supplement received during re-nutrition: milk, PFM or its bacterial-free supernatant (BFS). They were compared with well-nourished and malnourished mice. PFM was the most effective re-nutrition supplement to improve the histology of the thymus, decreasing cellular apoptosis in this organ and recovering the percentage of CD4(+)/CD8(-) single-positive thymocytes. Immature double-positive thymocytes were increased in the malnourished control (MC). The production of different cytokines in the thymus was increased in mice given PFM, compared with the mice that received other dietary supplements and MC. Mice given the BFS presented an improvement in the thymus similar to those that received milk. We demonstrated the importance of the whole PFM supplementation on the histological and functional recovery of the thymus in a non-severe PEM model.