Overexpression of Soybean Isoflavone Reductase (GmIFR) Enhances Resistance to Phytophthora sojae in Soybean.
Overexpression of Soybean Isoflavone Reductase (GmIFR) Enhances Resistance to Phytophthora sojae in Soybean.
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DOI：
10.3389/fpls.2015.01024
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发表时间：
2015
影响因子：
5.6
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Cheng Q;Li N;Dong L;Zhang D;Fan S;Jiang L;Wang X;Xu P;Zhang S
异黄酮还原酶(IFR)是植物体内异黄酮类植物保卫素生物合成途径中的一种酶。IFRS是植物界独有的，被认为在植物对各种生物和非生物环境胁迫的反应中发挥着至关重要的作用。在这里，我们报道了大豆异黄酮还原酶基因家族的一个新成员GmIFR的特征。过量表达GmIFR基因大豆表现出对大豆疫霉的抗性增强。胁迫处理后，大豆疫霉、乙烯利(ET)、脱落酸(PlaceCityABA)、水杨酸(SA)均显著诱导GmIFR。在大豆原生质体中瞬时表达时，它位于细胞质中。转基因植株种子中大豆苷元含量显著降低，而甘油三酯的相对含量显著高于非转基因植株。此外，我们还发现转基因大豆植株体内ROS的相对表达水平显著低于非转基因植株，这表明GmIFR可能作为一种重要的抗氧化剂来降低大豆体内的ROS。酶活性测定表明，GmIFR具有异黄酮还原酶活性。
Isoflavone reductase (IFR) is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin in plants. IFRs are unique to the plant kingdom and are considered to have crucial roles in plant response to various biotic and abiotic environmental stresses. Here, we report the characterization of a novel member of the soybean isoflavone reductase gene family GmIFR. Overexpression of GmIFR transgenic soybean exhibited enhanced resistance to Phytophthora sojae. Following stress treatments, GmIFR was significantly induced by P. sojae, ethephon (ET), abscisic acid (placeCityABA), salicylic acid (SA). It is located in the cytoplasm when transiently expressed in soybean protoplasts. The daidzein levels reduced greatly for the seeds of transgenic plants, while the relative content of glyceollins in transgenic plants was significantly higher than that of non-transgenic plants. Furthermore, we found that the relative expression levels of reactive oxygen species (ROS) of transgenic soybean plants were significantly lower than those of non-transgenic plants after incubation with P. sojae, suggesting an important role of GmIFR might function as an antioxidant to reduce ROS in soybean. The enzyme activity assay suggested that GmIFR has isoflavone reductase activity.