Galanin stimulates rat pituitary growth hormone secretion in vitro.
Galanin stimulates rat pituitary growth hormone secretion in vitro.
复制标题
甘丙肽在体外刺激大鼠垂体生长激素分泌。
DOI：
10.1016/0024-3205(88)90497-3
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发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Martin,JB
中科院分区：
文献类型：
--
作者：
Gabriel,SM;Milbury,CM;Nathanson,JA;Martin,JB
在大鼠垂体前叶细胞的单层培养物中研究了甘丙肽对生长激素（GH）分泌的影响。甘丙肽导致培养基中的 GH 浓度逐渐增加，在 90 分钟时达到最大，并在 180 分钟后持续。甘丙肽刺激的GH分泌的ED 50 约为200nM，相比之下，大鼠GH释放因子(rGRF)刺激的GH分泌的ED 50 为10pM。甘丙肽和rGRF 在增加孵化培养基中的GH 释放方面具有附加作用。这些数据表明，猪源性甘丙肽在体外对垂体 GH 分泌有直接影响。
The effect of galanin on growth hormone (GH) secretion was investigated in monolayer cultures of rat anterior pituitary cells. Galanin caused a gradual increase in GH concentrations into the culture medium that was maximal at 90 minutes and sustained after 180 minutes. The ED 50 for galanin-stimulated GH secretion was approximately 200nM compared to an ED 50 for rat GH-releasing factor (rGRF)-stimulated GH secretion of 10pM. Galanin and rGRF were additive in increasing GH release into the incubation medium. These data indicate that porcine-derived galanin has a direct effect on pituitary GH secretion in vitro.