Biological safety of fish (tilapia) collagen.
Biological safety of fish (tilapia) collagen.
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DOI：
10.1155/2014/630757
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发表时间：
2014
影响因子：
--
通讯作者：
Hayashi Y
中科院分区：
文献类型：
--
作者：
Yamamoto K;Igawa K;Sugimoto K;Yoshizawa Y;Yanagiguchi K;Ikeda T;Yamada S;Hayashi Y
来源于鱼鳞、皮肤和骨骼的海洋胶原蛋白具有良好的生物相容性、低抗原性、高生物降解性和细胞生长潜力等生物活性，因此被广泛研究用作支架和载体。鱼I型胶原蛋白是一种有效的材料，作为可生物降解的支架或间隔物复制天然细胞外基质，其用于空间组织细胞，为它们提供环境信号并指导位点特异性细胞调节。本研究的目的是确定鱼（罗非鱼）去端胶原在临床应用中的安全性。我们进行了体外和体内生物学研究的医疗材料，以探讨鱼胶原蛋白的安全性。对鱼胶原蛋白凝胶提取液进行了无菌性检查。根据ISO和日本厚生劳动省的标准，所有关于细菌和病毒（包括内毒素）的现有无菌试验均得出阴性结果，所有细胞毒性、致敏性、染色体畸变、皮内反应、急性全身毒性、热原反应和溶血的评价均为阴性。本研究表明，从罗非鱼中制备的去端胶原是一种很有前途的生物材料，可用作再生医学的支架。
Marine collagen derived from fish scales, skin, and bone has been widely investigated for application as a scaffold and carrier due to its bioactive properties, including excellent biocompatibility, low antigenicity, and high biodegradability and cell growth potential. Fish type I collagen is an effective material as a biodegradable scaffold or spacer replicating the natural extracellular matrix, which serves to spatially organize cells, providing them with environmental signals and directing site-specific cellular regulation. This study was conducted to confirm the safety of fish (tilapia) atelocollagen for use in clinical application. We performed in vitro and in vivo biological studies of medical materials to investigate the safety of fish collagen. The extract of fish collagen gel was examined to clarify its sterility. All present sterility tests concerning bacteria and viruses (including endotoxin) yielded negative results, and all evaluations of cell toxicity, sensitization, chromosomal aberrations, intracutaneous reactions, acute systemic toxicity, pyrogenic reactions, and hemolysis were negative according to the criteria of the ISO and the Ministry of Health, Labour and Welfare of Japan. The present study demonstrated that atelocollagen prepared from tilapia is a promising biomaterial for use as a scaffold in regenerative medicine.