Cyclophilin acts as a ribosome biogenesis factor by chaperoning the ribosomal protein (PlRPS15) in filamentous fungi.
Cyclophilin acts as a ribosome biogenesis factor by chaperoning the ribosomal protein (PlRPS15) in filamentous fungi.
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亲环蛋白通过陪伴丝状真菌中的核糖体蛋白 (PlRPS15) 作为核糖体生物发生因子
DOI：
10.1093/nar/gkab1102
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发表时间：
2021-12-02
影响因子：
14.9
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Mo C;Xie C;Wang G;Tian T;Liu J;Zhu C;Xiao X;Xiao Y
摘要核糖体蛋白快速转运到细胞核并有效地组装成前核糖体颗粒是核糖体生物合成的先决条件。在丝状真菌中还没有鉴定出作为RP的专用分子伴侣以维持其稳定性并促进其组装的蛋白质。PlCYP 5是淡紫紫孢菌（Purpureocillium lilacinum）的核亲环素，其表达在非生物胁迫和线虫卵寄生期间上调。在这里，我们发现PlCYP 5与未组装的小核糖体亚基蛋白PlRPS 15（uS 19）共-互补相互作用。PlRPS 15含有介导与PlCYP 5相互作用的真核生物特异性N-末端延伸。PlCYP 5独立于其催化肽脯氨酰异构酶功能增加了PlRPS 15的溶解度，并支持PlRPS 15整合到前核糖体中。一致地，PlCYP 5功能丧失突变体的表型与PlRPS 15敲低突变体的表型相似（例如生长和核糖体生物发生缺陷）。在拟南芥、智人、粟酒裂殖酵母、核盘菌、灰葡萄孢和绿僵菌中鉴定了PlCYP 5同源物。值得注意的是，来自三种丝状真菌的PlCYP 5-PlRPS 15同源物彼此相互作用，但来自其他物种的PlCYP 5-PlRPS 15同源物不相互作用。总之，我们的数据揭示了丝状真菌中亲环蛋白对RP的独特的专用伴侣系统。
Abstract The rapid transport of ribosomal proteins (RPs) into the nucleus and their efficient assembly into pre-ribosomal particles are prerequisites for ribosome biogenesis. Proteins that act as dedicated chaperones for RPs to maintain their stability and facilitate their assembly have not been identified in filamentous fungi. PlCYP5 is a nuclear cyclophilin in the nematophagous fungus Purpureocillium lilacinum, whose expression is up-regulated during abiotic stress and nematode egg-parasitism. Here, we found that PlCYP5 co-translationally interacted with the unassembled small ribosomal subunit protein, PlRPS15 (uS19). PlRPS15 contained an eukaryote-specific N-terminal extension that mediated the interaction with PlCYP5. PlCYP5 increased the solubility of PlRPS15 independent of its catalytic peptide-prolyl isomerase function and supported the integration of PlRPS15 into pre-ribosomes. Consistently, the phenotypes of the PlCYP5 loss-of-function mutant were similar to those of the PlRPS15 knockdown mutant (e.g. growth and ribosome biogenesis defects). PlCYP5 homologs in Arabidopsis thaliana, Homo sapiens, Schizosaccharomyces pombe, Sclerotinia sclerotiorum, Botrytis cinerea and Metarhizium anisopliae were identified. Notably, PlCYP5-PlRPS15 homologs from three filamentous fungi interacted with each other but not those from other species. In summary, our data disclosed a unique dedicated chaperone system for RPs by cyclophilin in filamentous fungi.
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在核糖体生物发生过程中，通过占据必需的rRNA结合位点，在核糖体生物发生过程中进行了1伴侣。
影响因子：
16.6
作者：
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作者：
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11.4
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通讯作者：
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作者：
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