Transcriptome Changes Induced by Botrytis cinerea Stress and Weighted Gene Co-expression Network Analysis (WGCNA) in Actinidia chinensis
Transcriptome Changes Induced by Botrytis cinerea Stress and Weighted Gene Co-expression Network Analysis (WGCNA) in Actinidia chinensis
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DOI：
10.1007/s11105-021-01325-3
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发表时间：
2022-01-04
影响因子：
2.1
通讯作者：
Tang, Jian-Min
中科院分区：
文献类型：
--
作者：
Li, Zhe-Xin;Zhang, Wen-Lin;Tang, Jian-Min
猕猴桃（Actinidia chinensis）富含膳食纤维和维生素C，具有很高的商业价值。然而，它很容易受到灰霉病的发病。这种真菌会引起灰霉病，对猕猴桃的品质产生负面影响。它还会引起超氧化物歧化酶、过氧化物酶和过氧化氢酶活性的变化。过氧化物酶和超氧化物歧化酶活性在1 dpi时检测到，直到4 dpi时增加，并在5 dpi时下降。过氧化氢酶在 2 dpi 时被激活，此后增加，并在 5 dpi 时下降。 RNA-seq 鉴定了 2726 个独特的差异表达基因。倍数变化至少为2倍，错误发现率小于0.01。与灰霉病反应相关的 233 个基因在前三个感染阶段受到调节。加权基因共表达网络分析确定了转录组数据集中对灰霉病有反应的基因和模块。网络分析揭示了“植物-病原体相互作用”、“脂质代谢”、“植物激素信号传导”、“转录因子”、“细胞壁生物发生”和“苯丙素生物合成”家族可能调节灰霉病菌反应。这里确定的中心基因是培育具有改善生物胁迫耐受性的奇异果的潜在目标。这项工作为研究猕猴桃中针对灰霉病发病机制进行调节的蛋白质奠定了经验基础。
Kiwifruit (Actinidia chinensis) is rich in dietary fiber and vitamin C and has high commercial value. However, it is susceptible to Botrytis cinerea pathogenesis. This fungus causes gray mold rot which negatively impacts kiwifruit quality. It also induces changes in superoxide dismutase, peroxidase, and catalase activity. Peroxidase and superoxide dismutase activities were detected at 1 dpi, increased till 4 dpi, and decreased by 5 dpi. Catalase was activated at 2 dpi, increased thereafter, and declined by 5 dpi. RNA-seq identified 2726 unique differentially expressed genes. The fold change was at least two, and the false discovery rate was less than 0.01. The 233 genes relevant to B. cinerea response were modulated in the first three infection stages. A weighted gene co-expression network analysis identified genes and modules in the transcriptome datasets that were responsive to B. cinerea. A network analysis disclosed the families "Plant-pathogen interactions", "Lipid metabolism", "Phytohormone signaling", "Transcription factor", "Cell wall biogenesis" and "Phenylpropanoid biosynthesis" possibly regulating B. cinerea response. The hub genes identified here are potential targets for breeding kiwifruit with improved biotic stress tolerance. This work lays an empirical foundation for investigating the proteins in kiwifruit that are modulated in response to B. cinerea pathogenesis.