Mouse model of sublethal and lethal intraperitoneal glanders (Burkholderia mallei)
Mouse model of sublethal and lethal intraperitoneal glanders (Burkholderia mallei)
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DOI：
10.1354/vp.37-6-626
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发表时间：
2000-11-01
影响因子：
2.4
通讯作者：
Waag, DM
中科院分区：
文献类型：
--
作者：
Fritz, DL;Vogel, P;Waag, DM
将60只雄性BALB/c小鼠分别接种亚致死量和致死量的马来伯克霍尔德氏菌中国7株，于接种后多个时间点处死小鼠。两组的组织病理学改变在性质上是相似的，包括化脓性肉芽肿性炎症。在亚致死性研究小鼠中，6h开始出现纵隔淋巴结的变化，随后在第3天出现脾、肝、外周淋巴结和骨髓的变化。这些变化一般在第4天达到最大发生率和严重程度，但与之相比，除肝脏外，所有组织的变化都有所减少。这种变化首先出现在致死性研究小鼠身上，6小时后还出现在纵隔淋巴结和脾内。在第1天，肝脏、外周淋巴结和骨髓出现变化。这些变化的发生率和严重程度在第2天达到最大。与亚致死性研究小鼠相比，在研究的其余部分，这些变化的发生率和严重程度并没有降低。两组之间最显著的区别是，在致命的研究小鼠中，脾迅速受累。表明血管灌注受损的变化更多地出现在亚致死性研究小鼠中。我们的发现表明，小鼠对马来芽胞杆菌感染很敏感，并可能成为抵抗宿主(如人类)腺体感染的合适模型。此外，通过免疫电子显微镜，我们发现在马来杆菌周围存在IO型抗原性多糖(囊膜)抗原。
Sixty male BALB/c mice were inoculated intraperitoneally with either a sublethal or a lethal dose of Burkholderia mallei China 7 strain, then killed at multiple time points postinoculation. Histopathologic changes were qualitatively similar in both groups and consisted of pyogranulomatous inflammation. In sublethal study mice, changes were first seen at 6 hours in mediastinal lymph nodes, then in spleen, liver, peripheral lymph nodes, and bone marrow at day 3. These changes generally reached maximal incidence and severity by day 4 but decreased by comparison in all tissues except the liver. Changes were first seen in lethal study mice also at 6 hours in mediastinal lymph nodes and in spleens. At day 1, changes were present in liver, peripheral lymph nodes, and bone marrow. The incidence and severity of these changes were maximal at day 2. In contrast to sublethal study mice, the incidence and severity of the changes did not decrease through the remainder of the study. The most significant difference between the two groups was the rapid involvement of the spleen in the lethal study mice. Changes indicative of impaired vascular perfusion were more frequently seen in the sublethal study mice. Our findings indicate that mice are susceptible to B. mallei infection and may serve as an appropriate model for glanders infection in a resistant host such as human beings. Additionally, by immunoelectron microscopy, we showed the presence of type I O-antigenic polysaccharide (capsular) antigen surrounding B. mallei.