Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
Direct Detection of Single Nucleotide Polymorphism (SNP) by the TaqMan PCR Assay Using Dried Saliva on Water-soluble Paper and Hair-roots, without DNA Extraction
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DOI：
10.2116/analsci.30.427
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发表时间：
2014-03-01
影响因子：
1.6
通讯作者：
Kinoshita, Kenji
中科院分区：
文献类型：
--
作者：
Hayashida, Mariko;Ota, Tomoko;Kinoshita, Kenji
我们已经开发了一种新的方法，直接使用未经处理的生物标本作为模板的TaqMan检测。DNA提取和纯化被认为是检测所必需的，但我们的新方法可以避免阻碍荧光检测，即使直接使用模板。需要将唾液放在水溶性纸上并干燥，并将毛发切成约10 mm长。这种方法可以减少所涉及的时间和精力，以及污染的风险。它应该被证明是非常有价值的基因诊断在各个领域。
We have developed a new method for directly using unprocessed biological specimens as templates for the TaqMan assay. DNA extraction and purification had been believed to be required for the assay, but our new method could avoid hindering fluorescence detection, even if the templates were used directly. Saliva was needed to be put on water-soluble paper and dried, and hairs were cut to be about 10 mm long. This method could reduce both the time and effort involved, and also the risk of contamination. It should prove to be very valuable for genetic diagnoses in various fields.