A COMPUTERIZED CALIBRATION OF CIRCULAR DICHROMETER
A COMPUTERIZED CALIBRATION OF CIRCULAR DICHROMETER
复制标题
DOI：
10.1021/bi00833a026
复制
发表时间：
1969-01-01
期刊：
影响因子：
2.9
通讯作者：
YANG, JT
中科院分区：
文献类型：
--
作者：
CASSIM, JY;YANG, JT
本文用最小二乘参数曲线拟合法对圆二色仪和标准化分光光度计进行了校准。通过测定两种樟脑衍生物在溶液中的“校准”摩尔圆二向色吸收c1-e n，证明了该技术的应用。最大cV_>圆二色性很容易成为溶液中生物聚合物构象研究的一种流行技术（参见例如Beychok，1968; Yang，1969; Yang和Samejima，1969）。它补充了在过去十年中几乎垄断了文献的旋光色散。几种商用圆二色仪现在能够在很宽的光谱范围内进行高精度测量。然而，由于缺乏一个普遍接受的标准，这些仪器的校准仍然存在不确定度。相反，分光偏振计可以用标准蔗糖溶液或已知旋光度的石英控制板校准。本文介绍了一种基于Thiéry（1968）开发的计算机程序的校准方法。实质上，该方法包括相对于经校准的分光偏振计校准圆二色性计。
A least-squares parametriccurve-fitting method is used tocalibrate a circular dichrometer against a standardized spectropolarimeter. An applica-tion of this technique is demonstrated by determining the “calibrated” molar circular dichoic absorption, ci—e „of two camphor derivatives in solution. Maximal cV_> ircular dichroism is readily becoming a popular technique for conformational studies of biopolymers in solution (see, for example, Beychok, 1968; Yang, 1969; Yang and Samejima, 1969). It complements optical rotatory dispersion which has virtually monopolized the literature over the last decade. Several commercial circular dichrometers are now capable of high-precision measurements over a wide region of the spectrum. How-ever, there is still uncertainty in the calibration of these instruments because of the lack of a universally accep-table standard. In contrast, spectropolarimeters can be calibrated with standard sucrose solutions or with quartz control plates of known rotations. In this com-munication we present a method of calibration based on a computer program developed by Thiéry (1968). In essence the method consists of calibrating the circular dichrometer against a calibrated spectropolarimeter.