A "whirly" transcription factor is required for salicylic acid-dependent disease resistance in Arabidopsis
A "whirly" transcription factor is required for salicylic acid-dependent disease resistance in Arabidopsis
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DOI：
10.1016/s1534-5807(04)00028-0
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发表时间：
2004-02-01
影响因子：
11.8
通讯作者：
Brisson, N
中科院分区：
文献类型：
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作者：
Desveaux, D;Subramaniam, R;Brisson, N
转录重编程是植物抗病反应的关键；人们对它的全球控制还不太了解。水杨酸（SA）可以诱导植物防御基因的表达，并形成一种长期的抗病状态，称为系统获得性抗性（SAR）。植物特异性的“Whirly”DNA结合蛋白先前与防御基因调控有关。我们证明了马铃薯StWhy1蛋白是含有pb2结合PB启动子元件的基因的转录激活因子。拟南芥StWhy1同源基因AtWhy1的DNA结合活性是由SA诱导的，是SA依赖的抗病和SA诱导的SAR应答基因表达所必需的。AtWhy1在完全的基础和特异性抗病反应中都是必需的。转录因子相关蛋白NPR1也是SAR所必需的。令人惊讶的是，SA激活AtWhy1是独立于NPR1的，这表明AtWhy1与NPR1一起转导SA信号。我们对AtWhyl的分析为依赖sa的植物抗病反应增加了一个关键成分。
wTranscriptional reprogramming is critical for plant disease resistance responses; its global control is not well understood. Salicylic acid (SA) can induce plant defense gene expression and a long-lasting disease resistance state called systemic acquired resistance (SAR). Plant-specific "Whirly" DNA binding proteins were previously implicated in defense gene regulation. We demonstrate that the potato StWhy1 protein is a transcriptional activator of genes containing the PBF2 binding PB promoter element. DNA binding activity of AtWhy1, the Arabidopsis StWhy1 ortholog, is induced by SA and is required for both SA-dependent disease resistance and SA-induced expression of an SAR response gene. AtWhy1 is required for both full basal and specific disease resistance responses. The transcription factor-associated protein NPR1 is also required for SAR. Surprisingly, AtWhy1 activation by SA is NPR1 independent, suggesting that AtWhy1 works in conjunction with NPR1 to transduce the SA signal. Our analysis of AtWhyl adds a critical component to the SA-dependent plant disease resistance response.