Time course of neuroanatomical and functional recovery after bilateral pudendal nerve injury in female rats
Time course of neuroanatomical and functional recovery after bilateral pudendal nerve injury in female rats
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DOI：
10.1152/ajprenal.00176.2007
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发表时间：
2007-11-01
影响因子：
4.2
通讯作者：
Kerns, James M.
中科院分区：
文献类型：
--
作者：
Damaser, Margot S.;Samplaski, Mary K.;Kerns, James M.
阴部神经支配尿道外括约肌（EUS），是分娩过程中受损的组织之一，可能导致压力性尿失禁（SUI）的症状。为了了解损伤和修复的机制，在双侧阴部神经挤压后4天、2周和6周测量尿道漏尿点压力（LPP）。通过光镜和电镜检查远端神经和EUS的形态学变化。为了确定是否恢复导致阴部神经再生，LPP测量之前和之后阴部神经切断后2周神经挤压。与假损伤动物相比，阴部神经挤压后4天以及神经挤压后2或6周，LPP显著降低。LPP是没有显着差异2或6周后神经压榨与假损伤的动物相比，表明尿道功能已恢复正常。阴部神经挤压后4天，损伤部位远端阴部神经的EUS分支显示神经变性的证据，EUS出现中断。神经夹伤后2周，远端神经和EUS均显示神经变性和恢复的证据。神经夹伤后2周，LPP在神经切断后显著降低。神经损伤后6周，在阴部神经和EUS中观察到神经再生的证据。这项研究表明，功能恢复和神经再生是显着的神经挤压后2周，虽然通过解剖评估，恢复似乎不完全，这表明2周代表了早期的时间点的初始神经再生。
The pudendal nerve innervates the external urethral sphincter (EUS) and is among the tissues injured during childbirth, which may lead to symptoms of stress urinary incontinence (SUI). To understand the mechanisms of injury and repair, urethral leak-point pressure (LPP) was measured 4 days, 2 wk, or 6 wk after bilateral pudendal nerve crush. Morphometric changes in the distal nerve and EUS were examined by light and electron microscopy. To determine whether recovery resulted from pudendal neuroregeneration, LPP was measured before and after pudendal nerve transection 2 wk after nerve crush. LPP was significantly decreased 4 days after pudendal nerve crush compared with sham-injured animals as well as 2 or 6 wk after nerve crush. LPP was not significantly different 2 or 6 wk after nerve crush compared with sham-injured animals, suggesting that urethral function had returned to normal. Four days after pudendal nerve crush, the EUS branch of the pudendal nerve distal to the injury site showed evidence of nerve degeneration and the EUS appeared disrupted. Two weeks after nerve crush, the distal nerve and EUS both showed evidence of both nerve degeneration and recovery. Two weeks after nerve crush, LPP was significantly decreased after nerve transection. Six weeks after nerve injury, evidence of neuroregeneration was observed in the pudendal nerve and the EUS. This study has demonstrated that functional recovery and neuroregeneration are significant 2 wk after nerve crush, although by anatomical assessment, recovery appears incomplete, suggesting that 2 wk represents an early time point of initial neuroregeneration.