Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation.
Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation.
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花生凝集素与乳腺上皮、人类癌症和培养的大鼠乳腺干细胞的结合：使用凝集素作为乳腺分化的标志物。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Rudland
中科院分区：
文献类型：
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作者：
R. Newman;P. Klein;P. Rudland
我们研究了荧光标记的花生凝集素（PNA）的乳腺上皮细胞的结合。特异性结合的PNA的雌性SD大鼠乳腺的腺体成熟增加。性不成熟的大鼠表现出相对较少的荧光，但这在成熟和怀孕的动物增加。在哺乳期大鼠中达到最大值，其中观察到管腔内材料的显著标记。PNA仅与上皮细胞结合，而不与肌上皮细胞或间充质细胞结合。在组织培养中，大鼠乳腺上皮干细胞系，它可以被刺激分化为肺泡样分泌细胞或肌上皮细胞，显示证据的PNA结合仅在分泌细胞上，而不是在未受刺激的或肌上皮细胞。成纤维细胞培养物也未能显示出PNA的显著结合。分泌细胞上的受体位点主要被唾液酸掩蔽。人类乳房切片，像那些的大鼠，在上皮细胞的顶端区域显示荧光标记;这种标记增加，如果组织有预先治疗与神经氨酸酶。形态分化的乳腺癌显示更多的标记与PNA比未分化的肿瘤，往往有弱或有时负标记。当观察到显著的荧光时，其主要位于细胞质中。相比之下，标记仅限于分化癌的细胞周边。因此，提出使用PNA作为乳腺上皮细胞分化的标志物。
We investigated the binding of fluorescence-labeled peanut agglutinin (PNA) to breast epithelium. Specific binding of PNA to the mammary glands of female Sprague-Dawley rats increased as the gland matured. Sexually immature rats showed relatively little fluorescence, but this increased in mature and pregnant animals. A maximum was reached in lactating rats in which significant labeling of material within the lumen was observed. PNA was bound exclusively to the epithelial and not the myoepithelial or mesenchymal cells. In tissue culture, a rat mammary epithelial stem cell line, which can be stimulated to differentiate to alveolus-like secretory or myoepithelial cells, showed evidence of PNA binding only on the secretory cells and not on unstimulated or myoepithelial cells. Fibroblast cultures also failed to show significant binding of PNA. Receptor sites on the secretory cells were masked mainly by sialic acid. Human breast sections, like those of the rat, showed fluorescent labeling at the apical region of the epithelial cells; this labeling increased if the tissue had prior treatment with neuraminidase. Breast carcinomas that were morphologically differentiated showed more labeling with PNA than did undifferentiated tumors, which often had weak or sometimes negative labeling. When significant fluorescence was observed, it was localized mainly in the cytoplasm. By contrast, labeling was restricted to the cell periphery in differentiated carcinomas. The use of PNA as a marker for breast epithelial cell differentiation is therefore proposed.