Highly sensitive detection of Staphylococcus aureus by a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification.
Highly sensitive detection of Staphylococcus aureus by a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification.
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基于金纳米粒子和滚环放大的太赫兹超材料生物传感器高灵敏检测金黄色葡萄球菌
作者：
迫切需要一种检测金黄色葡萄球菌(金黄色葡萄球菌)的高灵敏度方法，以减少医院感染和食源性疾病的影响和传播。为此，本文提出了一种基于纳米金(AuNPs)和滚环放大(RCA)的太赫兹超材料生物传感器。RCA过程扩增了金黄色葡萄球菌的DNA片段，并产生了大量的长单链DNA分子。然后，这些分子与AuNPs形成络合物，使样品的折射率异常增加，并导致超材料的THz响应得到相应的改善。在最佳条件下，超材料的共振频率与合成金黄色葡萄球菌DNA的浓度在10 fM-10 pm之间呈线性关系，检测下限为2.77 fM。我们还测试了该传感器在临床菌株基因组DNA检测中的实际应用，该传感器的检出限为0.08pgμL−1，线性范围为0.1~5pgμL−1，并具有较好的特异性，能抵抗其他三种致病菌的干扰。这些发现表明，所提出的方法提供了一种经济有效的太赫兹生物传感策略，该策略可以容易地制造和操作，以帮助诊断传染病和食品安全控制。迫切需要一种检测金黄色葡萄球菌(金黄色葡萄球菌)的高灵敏度方法，以减少医院感染和食源性疾病的影响和传播。
A highly sensitive method for detecting Staphylococcus aureus (S. aureus) is urgently needed to reduce the impact and spread of hospital-acquired infections and food-borne illness. For this purpose, this paper presents a THz metamaterial biosensor based on gold nanoparticles (AuNPs) and rolling circle amplification (RCA). The RCA process amplified the S. aureus DNA fragments and generated copious yields of long single-strand DNA molecules. These molecules were then conjugated with the AuNPs to form complexes that delivered exceptional increases in the refractive indices of the samples, and resulted in corresponding improvements in the THz response of the metamaterial. Under optimal conditions, the shifts in the metamaterial's resonance frequency displayed a linear relationship with concentrations of synthetic S. aureus DNA in the range from 10 fM to 10 pM, with a limit of detection of 2.77 fM. We also tested the practical application of this biosensor in measurements of genomic DNA in clinical bacterial strains, where the sensor showed a detection limit of 0.08 pg μL−1 and a linear range from 0.1 to 5 pg μL−1. It also exhibited reasonable specificity, resisting interference from three other pathogenic bacteria. These findings indicate that the proposed approach offers a cost-effective THz biosensing strategy that can be easily fabricated and conveniently operated to aid the diagnosis of infectious disease and food safety control. A highly sensitive method for detecting Staphylococcus aureus (S. aureus) is urgently needed to reduce the impact and spread of hospital-acquired infections and food-borne illness.
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影响因子：
4.6
作者：
Hong, J. T.;Jun, S. W.;Ahn, Y. H.
通讯作者：
Ahn, Y. H.
影响因子：
12.6
作者：
Sayad, Abkar;Ibrahim, Fatimah;Kwai Lin Thong
通讯作者：
Kwai Lin Thong
影响因子：
12.6
作者：
Torres-Chavolla, Edith;Alocilja, Evangelyn C.
通讯作者：
Alocilja, Evangelyn C.
DOI：
10.1016/j.saa.2018.03.056
发表时间：
2018-06-15
影响因子：
4.4
作者：
Amini, Bahram;Kamali, Mehdi;Yaghmaei, Parichehreh
通讯作者：
Yaghmaei, Parichehreh
影响因子：
4.6
作者：
Lee DK;Kang JH;Lee JS;Kim HS;Kim C;Kim JH;Lee T;Son JH;Park QH;Seo M
通讯作者：
Seo M