Transgene driving GFP expression from the promoter of the zona pellucida gene zpc is expressed in oocytes and provides marker for gonad differentiation in an early zebrafish
Transgene driving GFP expression from the promoter of the zona pellucida gene zpc is expressed in oocytes and provides marker for gonad differentiation in an early zebrafish
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DOI：
10.1002/dvdy.10392
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发表时间：
2003-11-01
影响因子：
2.5
通讯作者：
Driever, W
中科院分区：
文献类型：
--
作者：
Onichtchouk, D;Aduroja, K;Driever, W
尽管哺乳动物、昆虫和蠕虫的性别分化机制已经被深入研究，但对低等脊椎动物的性别分化过程知之甚少。为了建立斑马鱼雌性性腺分化的标记，我们构建了一个转基因品系，其中雌性特异性透明带基因zpc的启动子和5' mRNA前导序列的412 bp与绿色荧光蛋白（GFP）的编码区融合。zpc0.5.GFP转基因仅在卵母细胞中表达，从雌性特异性分化开始，并且与野生型zpc的表达模式非常相似。强烈的绿色荧光蛋白表达持续整个卵子发生，是可见的，通过体壁的女性。我们还确定了一个假定的上游因子ZPC，FIGaIpha，并表明FIGaIpha RNA的分布与其在ZPC调节中的假定作用是相容的。本文所述的zpc0.5.GFP转基因品系将有助于研究斑马鱼卵母细胞发育和性别特异性基因表达的机制。它也是迄今为止第一个被鉴定为在斑马鱼雌性生殖系中特异性地驱动稳定和有效表达的启动子。(C)2003 Wiley-Liss，Inc.
Although mechanisms of sex differentiation have been studied intensely in mammals, insects, and worms, little is known about this process in lower vertebrates. To establish a marker for female gonad differentiation in zebrafish, we generated a transgenic line in which 412 bp from the promoter and 5' mRNA leader of the female-specific zebrafish zona pellucida gene zpc are fused to the coding region of green fluorescent protein (GFP). The zpc0.5.GFP transgene is expressed exclusively in oocytes, starting from the onset of female-specific differentiation, and closely resembles the expression pattern of the wild-type zpc. Strong GFP expression persists throughout oogenesis and is visible through the body wall of females. We have also characterized a putative upstream factor of zpc, FIGaIpha, and show that distribution of FIGaIpha RNA is compatible with its postulated role in the regulation of zpc. The zpc0.5.GFP transgenic line described here will be useful for studying oocyte development and the mechanisms that determine sex-specific gene expression in the zebrafish. It is also the first promoter characterized to date to drive stable and efficient expression specifically in the zebrafish female germline. (C) 2003 Wiley-Liss, Inc.