Tea polyphenols alleviate tri-ortho-cresyl phosphate-induced autophagy of mouse ovarian granulosa cells
Tea polyphenols alleviate tri-ortho-cresyl phosphate-induced autophagy of mouse ovarian granulosa cells
复制标题
茶多酚减轻磷酸三邻甲苯酯诱导的小鼠卵巢颗粒细胞自噬
DOI：
10.1002/tox.22883
复制
发表时间：
2019-12-02
影响因子：
4.5
通讯作者：
Wang，Jinglei
中科院分区：
文献类型：
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作者：
Yang，Si;Shao，Shuxin;Wang，Jinglei
三邻甲酚磷酸(TOCP)是一种工业上广泛使用的增塑剂，可导致女性生殖损害。茶多酚(TPS)通过抑制氧化应激对人体健康有多种影响。然而，TPS对TOCP所致女性生殖系统损伤的生殖保护作用尚不清楚。在本研究中，TOCP抑制了小鼠卵巢颗粒细胞的活力并诱导了自噬，而TPS可以挽救对细胞活力的抑制和对自噬的诱导。自噬抑制剂3-MA也能解除对细胞活力的抑制。这些结果表明，TPS对TOCP诱导的自噬具有保护作用。此外，TPS还能抑制TOCP诱导的细胞氧化应激，提示TPS可能通过抑制氧化应激来减轻TOCP诱导的自噬。此外，TPS还能挽救TOCP诱导的小鼠卵巢组织的自噬和氧化应激。综上所述，TPS对TOCP诱导的卵巢损伤有保护作用，其机制可能与抑制氧化应激有关。
Tri‐ortho‐cresyl phosphate (TOCP), a widely used plasticizer in industry, can cause female reproductive damage. Tea polyphenols (TPs) have multiple health effects via inhibiting oxidative stress. However, the reproductive protection of TPs in TOCP‐induced female reproductive system damage is yet to be elucidated. In the study, TOCP inhibited cell viability and induced autophagy of mouse ovarian granulosa cells; while TPs could rescue the inhibition of viability and induction of autophagy. 3‐MA, an autophagy inhibitor, could also rescue the inhibition of cell viability. These results indicated that TPs played a protective role in TOCP‐induced autophagy. Furthermore, TPs could inhibit the induction of oxidative stress of the cells by TOCP, which implying that TPs might alleviate TOCP‐induced autophagy via inhibiting oxidative stress. Furthermore, TPs could rescue TOCP‐induced autophagy and oxidative stress in the mouse ovarian tissues. Taken together, these results indicated that TPs could protect TOCP‐induced ovarian damage via inhibiting oxidative stress.