Re-evaluation of nestin as a marker of oligodendrocyte lineage cells
Re-evaluation of nestin as a marker of oligodendrocyte lineage cells
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DOI：
10.1002/jemt.1060
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发表时间：
2001-03-15
影响因子：
2.5
通讯作者：
Guaza, C
中科院分区：
文献类型：
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作者：
Almazán, G;Vela, JM;Guaza, C
少突胶质细胞前体细胞(O2A)的成熟以形态变化和特定抗原的顺序表达为特征，从而导致髓鞘膜的形成。单抗A2B5、A007、抗波形蛋白和抗半乳糖脑苷能识别不同发育阶段的少突胶质细胞。神经上皮细胞前体标记Nestin也由少突胶质细胞谱系表达；我们使用从新生大鼠脑培养中分离的丰富的前体细胞群来进一步检测这种中间丝蛋白的细胞分布。Nestin阳性细胞在少突胶质细胞谱系中的表型分布显示，65%的细胞与A2B5反应，仅5%的细胞与A007(+)反应，4%的细胞与半乳糖脑苷脂(+)反应。剩下的25%的细胞没有标记，有小的细胞体，没有突起，这是O2A前体的特征。对巢蛋白(+)群体的进一步分析表明，大多数细胞也是波形蛋白(+)。抗体依赖补体介导的A2B5(+)(O2a细胞)和半乳脑苷脂(+)(成熟少突胶质细胞)细胞溶解后，留下一群巢蛋白(+)细胞，在生长因子的存在下被诱导增殖并分化为A2B5(+)和半乳脑苷脂(+)细胞。在血小板衍生生长因子或碱性成纤维细胞生长因子存在下维持的增殖细胞保留了巢蛋白和A2B5的表达。相反，在无血清培养中，巢蛋白表达减少，而有丝分裂后细胞获得A007和半乳糖脑苷类。我们的结果表明，巢蛋白的表达是前O2A细胞的一个标志，在增殖的神经胶质前体细胞中维持，但在有丝分裂后的少突胶质细胞(A007(+)/半乳糖脑苷脂(+))以及A2B5和Vimentin中迅速下调。然而，包括2型星形胶质细胞和一些阿米巴样小胶质细胞在内的其他胶质细胞也有巢蛋白的表达。(C)2001年Wiley-Liss，Inc.
Maturation of oligodendrocyte progenitors (O2A) is characterized by morphological changes and the sequential expression of specific antigens leading to the formation of myelin membrane. Monoclonal antibodies A2B5, A007, anti-vimentin, and anti-galactocerebroside, recognize oligodendroglia at different stages of development. The neuroepithelial precursor marker nestin is also expressed by the oligodendroglial lineage; we have used enriched populations of progenitors isolated from neonatal rat brain cultures to further examine the cellular distribution of this intermediate filament protein. The phenotypic distribution of nestin positive cells among the oligodendrocyte Lineage showed that 65% reacted with A2B5, whereas only 5% were A007(+), and 4% galactocerebroside(+)'. The remaining 25% of the cells were not labeled and had small cellular bodies devoid of processes, characteristic of the pre-O2A progenitor. Further analysis of the nestin(+) population showed that the majority of the cells were also vimentin(+). Antibody-dependent complement mediated cytolysis of A2B5(+) (O2A cells) and galactocerebroside(+) (mature oligodendrocytes) cells left a population of nestin(+) cells that were induced to proliferate in the presence of growth factors and to differentiate into A2B5(+) and galactocerebroside(+) cells. Proliferating cells maintained in the presence of platelet-derived growth factor or basic fibroblast growth factor retained nestin expression along with A2B5. By contrast, in serum-free medium nestin expression decreased while postmitotic cells acquired A007 and galactocerebroside. Our results suggest that nestin expression is a marker of pre-O2A cells that is maintained in proliferating glial progenitors, but is quickly down-regulated in postmitotic oligodendrocytes (A007(+)/galacto-cerebroside(+)) along with A2B5 and vimentin. However, other glial cells including type 2 astrocytes and some amoeboid microglia also share nestin expression. (C) 2001 Wiley-Liss, Inc.