ESTIMATION OF HYDROGEN-PEROXIDE FORMED AND RESIDUAL ASCORBATE IN THE COPPER CATALYZED OXIDATION REACTION OF ASCORBATE AT PH-7
ESTIMATION OF HYDROGEN-PEROXIDE FORMED AND RESIDUAL ASCORBATE IN THE COPPER CATALYZED OXIDATION REACTION OF ASCORBATE AT PH-7
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DOI：
10.1007/bf01242085
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发表时间：
1992-01-01
期刊：
影响因子：
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通讯作者：
COOPER, L
中科院分区：
文献类型：
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作者：
BAKER, WL;GOODE, J;COOPER, L
已经开发出一种荧光方法来测定抗坏血酸浓度。该方法包括用Cu(II)处理抗坏血酸，并在对羟基苯乙酸存在下处理用辣根过氧化物酶形成的过氧化氢，以形成荧光对羟基苯乙酸二聚体。该反应适用于分析浓度范围为 50-mu-M 至 4 mM 的抗坏血酸溶液，并且可用于分析药物制剂，但不适用于分析天然来源制剂中的抗坏血酸盐。通过对分析技术进行轻微修改，可以测量用不同量的铜离子处理的溶液中形成的过氧化氢的量和抗坏血酸的残留浓度。
A fluorescent method has been developed for determination of ascorbic acid concentrations. The method involves treatment of the ascorbic acid with Cu(II) and treating the hydrogen peroxide formed with horseradish peroxidase in the presence of p-hydroxyphenylacetic acid, to form a fluorescent p-hydroxyphenylacetic acid dimer. The reaction is suitable for analysis of concentrations in the range from 50-mu-M to 4 mM ascorbic acid solutions and can be used for analysis of pharmaceutical preparations but is unsuitable for analysis of ascorbate in preparations derived from natural sources. By using a slight modification of the analytical technique it is possible to measure the amount of hydrogen peroxide formed and the residual concentrations of ascorbic acid in solutions treated with varying amounts of copper ion.