ANALYSIS OF PROTEINS SYNTHESIZED BY SALMONELLA-TYPHIMURIUM DURING GROWTH WITHIN A HOST MACROPHAGE
ANALYSIS OF PROTEINS SYNTHESIZED BY SALMONELLA-TYPHIMURIUM DURING GROWTH WITHIN A HOST MACROPHAGE
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DOI：
10.1128/jb.175.12.3734-3743.1993
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发表时间：
1993-06-01
影响因子：
3.2
通讯作者：
NEIDHARDT, FC
中科院分区：
文献类型：
--
作者：
ABSHIRE, KZ;NEIDHARDT, FC
鼠伤寒沙门氏菌是一种兼性细胞内病原体，能够入侵真核细胞并在真核细胞内生存，并在各种细胞外环境中生长。为了比较细菌对这些不同环境的反应，并阐明细胞内环境的性质，我们通过二维聚丙烯酰胺凝胶电泳研究了蛋白质合成的模式。在巨噬细胞样U937细胞内生长期间，观察到约40种蛋白质的水平增加，而约100种蛋白质的水平相对于细胞外对照培养物的水平被抑制。为了帮助解释这些结果，S.鼠伤寒沙门氏菌暴露于各种环境条件下，在实验室进行了测定。然后将细胞内蛋白质模式与这些基准蛋白质模式中的每一个进行比较。该分析显示，正如预期的那样，细胞内环境似乎对细菌施加了许多应力，但出乎意料的是，巨噬细胞诱导的反应不是细胞外生长期间显示的单个应力反应的简单总和。
Salmonella typhimurium is a facultative intracellular pathogen, able both to invade and to survive within eukaryotic cells and to grow in various extracellular environments. To compare the bacterial responses to these disparate environments and to shed light on the nature of the intracellular environment, we have examined the pattern of protein synthesis by two-dimensional polyacrylamide gel electrophoresis. The levels of approximately 40 proteins were observed to increase during growth within macrophage-like U937 cells, while approximately 100 proteins exhibited levels that were repressed relative to those of an extracellular control culture. To aid in the interpretation of these results, the patterns of proteins made by S. typhimurium exposed to various environmental conditions in the laboratory were determined. The intracellular protein pattern was then compared with each of these benchmark protein patterns. This analysis revealed that, as expected, the intracellular environment appears to impose numerous stresses on the bacteria, but unexpectedly, the macrophage-induced response was not a simple sum of the individual stress responses displayed during extracellular growth.