CHARACTERIZATION OF SITES WITH ELEVATED LDL PERMEABILITY AT INTERCOSTAL, CELIAC, AND ILIAC BRANCHES OF THE NORMAL RABBIT AORTA
CHARACTERIZATION OF SITES WITH ELEVATED LDL PERMEABILITY AT INTERCOSTAL, CELIAC, AND ILIAC BRANCHES OF THE NORMAL RABBIT AORTA
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DOI：
10.1161/01.atv.14.2.313
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发表时间：
1994-02-01
期刊：
影响因子：
--
通讯作者：
TRUSKEY, GA
中科院分区：
文献类型：
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作者：
HERRMANN, RA;MALINAUSKAS, RA;TRUSKEY, GA
应用内皮放射自显影技术对正常兔主动脉肋间动脉、腹主动脉和髂动脉分叉处的I-125-低密度脂蛋白(I-125-LDL)通透性增强部位的分布、面积和通透性进行了定量研究。这些部位的密度在胸主动脉上段及腹主动脉和肠系膜上支周围最高，在腹主动脉下段最低。通透性部位多发生在肋间支口远端，以及腹侧支口的外侧和远侧。在肋间支口，这些部位较大，通透性较低，频率较高。在腹主动脉血流分隔处，放射自显影颗粒密度升高的部位比腹主动脉的其他部位更具渗透性且更大。区域平均渗透率由低渗透率和高渗透率场地的加权面积平均值得到。肋间支周围的平均区域渗透率是远离肋间支的1.5倍。在距分流板0.25 mm和1 mm范围内，平均通透性分别是远离分流板的3.1倍和1.3倍。在腹主动脉-髂动脉分叉远端仅见少量通透性增高的部位，髂动脉内侧壁和外侧壁的通透性无明显差异。有丝分裂细胞与对I-125-LDL通透性升高的所有部位中的13+/-8%相关。有丝分裂内皮细胞在分支部位的频率没有增加，这表明除了细胞复制之外的机制导致了兔低密度脂蛋白通透性的增加。这些结果表明，高胆固醇饮食兔的腹膜和肋间支周围通透性增高部位的通透性和出现频率可能影响早期病变的分布和严重程度。
En face autoradiography of the endothelium was used to quantify the distribution, area, and permeability of sites with enhanced permeability to I-125-low-density lipoprotein (I-125-LDL) around the intercostal and celiac arteries and at the iliac bifurcation of normal rabbit aortas. The density of such sites was highest in the upper thoracic aorta and around the celiac and superior mesenteric branches and was lowest in the lower abdominal aorta. Permeable sites occurred more frequently distal to the intercostal branch orifices and both lateral and distal to the orifice at the celiac branch. At the intercostal branch orifices, these sites were larger, with a lower permeability and higher frequency than those away from the branch. At the celiac flow divider, sites of elevated autoradiographic grain density were more permeable and larger than at other locations in the abdominal aorta. Mean regional permeabilities were obtained by weighted area averages of low- and high-permeability sites. Mean regional permeabilities around the intercostal branches were 1.5 times higher than values away from the intercostal branches. Within 0.25 and 1 mm away from the celiac flow divider, mean regional permeability was 3.1 and 1.3 times higher, respectively, than those away from the flow divider. Few sites of elevated permeability were found distal to the aortoiliac bifurcation, and the permeabilities at the medial and lateral walls of the iliac arteries were not different. Mitotic cells were associated with 13+/-8% of all sites with elevated permeability to I-125-LDL. The frequency of mitotic endothelial cells was not increased at branch sites, suggesting that mechanisms other than cell replication were responsible for increased LDL permeability in the rabbit. These results suggest that the permeability and frequency of occurrence of sites with elevated permeability around the celiac and intercostal branches may influence the distribution and severity of early lesions in rabbits fed a hypercholesterolemic diet.