The RNA polymerase II C-terminal domain A new role in spliceosome assembly
The RNA polymerase II C-terminal domain A new role in spliceosome assembly
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DOI：
10.4161/trns.2.5.17272
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发表时间：
2011-01-01
影响因子：
3.6
通讯作者：
Manley, James L.
中科院分区：
文献类型：
--
作者：
David, Charles J.;Manley, James L.
过去二十年的研究提供了大量数据，表明RNA聚合酶II的转录机制可以在促进其转录本的剪接方面发挥重要作用。特别是，RNA聚合酶II大亚基(CTD)的C末端结构域在转录和剪接的耦合过程中起着中心作用。虽然长期以来人们认为这涉及剪接因子和CTD之间的物理相互作用，但CTD和这些因子之间的功能联系还很少。我们最近使用了一种生化方法来确定一个剪接因子，它直接与CTD相互作用来激活剪接，并在这样做的过程中，可能在剪接体组装过程中发挥作用。
Work over the last two decades has provided a wealth of data indicating that the RNA polymerase II transcriptional machinery can play an important role in facilitating the splicing of its transcripts. In particular, the C-terminal domain of the RNA polymerase II large subunit (CTD) is central in the coupling of transcription and splicing. While this has long been assumed to involve physiccal interactions between splicing factors and the CTD, few functional connections between the CTD and such factors have been established. We recently used a biochemical approach to identify a splicing factor that interacts directly with the CTD to activate splicing and, in doing so, may play a role in the process of spliceosome assembly.