Parasites flicking the NPY gene on the host's switchboard: why NPY?
Parasites flicking the NPY gene on the host's switchboard: why NPY?
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DOI：
10.1096/fasebj.13.14.1972
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发表时间：
1999-11-01
期刊：
影响因子：
4.8
通讯作者：
Ter Maat, A
中科院分区：
文献类型：
--
作者：
De Jong-Brink, M;Reid, CN;Ter Maat, A
研究了在其蜗牛寄主停滞淋巴（lynaea stagnation）中，毛叉血吸虫（Trichobilharzia ocellata）上调NPY基因是否会改变寄主的能量流。我们克隆了lynaea NPY （LyNPY）的cDNA，对其进行了纯化和测序，并利用合成的肽进行了生理和形态学研究。通过1)植入缓释微丸和2)注射来提高未被寄生蜗牛（模拟寄生虫）的LyNPY滴度，在不影响食物摄入量的情况下，以剂量和时间依赖的方式抑制了繁殖活性和生长。当LyNPY滴度恢复正常时，繁殖和生长恢复，同时短暂增加食物摄入量以补充糖原储存，双免疫染色的全脑制剂支持这些数据。lynpy阳性轴突与排卵激素产生神经元和参与生长调节的软体动物胰岛素样肽产生神经元的轴突密切相关。由于没有观察到突触（类）接触，因此假设LyNPY不是asynapically起作用的。lynpy阳性轴突与支配摄食器的运动神经元之间没有形态上的相互作用。我们的数据解释了为什么内寄生虫上调宿主体内高度保守的NPY基因是一种有利的策略。-de Jong-Brink， M, Reid， C. N., Tensen， C. P., Ter Maat， A.寄生虫在宿主的交换机上轻敲NPY基因：为什么是NPY？
It was investigated whether up-regulation of the NPY gene by the schistosome Trichobilharzia ocellata in its snail host Lymnaea stagnalis redirects the host's energy flows. We cloned the cDNA encoding Lymnaea NPY (LyNPY), purified and sequenced the peptide, and used synthesized peptide for physiological and morphological studies. Increasing the LyNPY titer in nonparasitized snails (mimicking parasitosis) by 1) implantation of slow-release pellets and 2) injections suppressed reproductive activity and reduced growth in a dose- and time-dependent manner without affecting food intake. When the LyNPY titer was back to normal, reproduction and growth were resumed, coinciding with a transient increase of food intake serving to replenish glycogen stores, Observations on double-immunostained whole mount preparations of brains support these data. A close association was found between LyNPY-positive axons and axons both from ovulation hormone-producing neurons and molluscan insulin-like peptide-producing neurons involved in regulation of growth. As no synaptic(-like) contacts were observed, it is supposed that LyNPY acts noasynaptically. No morphological interaction was found between LyNPY-positive axons and motoneurons innervating the feeding apparatus. Our data explain why it is an advantageous strategy for endoparasites to up-regulate the highly conserved NPY gene in their host.-de Jong-Brink, M., Reid, C. N., Tensen, C. P., Ter Maat, A. Parasites flicking the NPY gene on the host's switchboard: why NPY?.