Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea)
Coordinated regulation of anthocyanin biosynthesis through photorespiration and temperature in peach (Prunus persica f. atropurpurea)
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DOI：
10.1007/s11295-012-0552-1
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发表时间：
2012-08
影响因子：
2.4
通讯作者：
Ying Zhou;D. Guo;Jing Li;Jun Cheng;Hui Zhou;C. Gu;Sue Gardiner;Yuepeng Han
中科院分区：
文献类型：
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作者：
Ying Zhou;D. Guo;Jing Li;Jun Cheng;Hui Zhou;C. Gu;Sue Gardiner;Yuepeng Han
桃（Prunus persicaf.atropurpurea）嫩叶的红色是由于花色素苷积累所致。实时荧光定量PCR分析结果表明，不同发育时期叶片中花青素生物合成基因的表达水平与花青素含量之间存在较强的相关性。与对照相比，花色素苷生物合成基因和光呼吸基因的表达谱在黑暗或热胁迫下均发生了显著变化。暗处理和高温处理后，桃红色叶片中花青苷生物合成基因的表达量显著下降，导致花青苷积累量显著减少。然而，光呼吸相关基因GDCH和GOX在黑暗或热处理后的桃叶中表达增加。此外，不积累花青素的拟南芥chi/f3′ h突变体中GDCH和GOXin的表达水平高于野生型。这些结果支持了光呼吸相关基因可能参与花青素合成调控的假设。这一发现为我们理解植物花色素苷生物合成的控制机制提供了新的见解。
The usual red color of young leaves of peach (Prunus persicaf.atropurpurea) is due to the accumulation of anthocyanin. Real-time PCR analysis revealed a strong correlation between the expression levels of anthocyanin biosynthetic genes and anthocyanin content in leaves at different developmental stages. The expression profiles of both anthocyanin biosynthetic genes and photorespiratory genes showed significant changes in leaves held in the dark or exposed to heat stress, compared with controls. The expression of anthocyanin biosynthetic genes dramatically decreased in peach red leaves following dark or heat treatments, resulting in a significant decrease of anthocyanin accumulation. However, the photorespiration-related genesGDCHandGOXexhibited increased expression in peach leaves after dark or heat treatment. Moreover, the expression levels ofGDCHandGOXin theArabidopsis chi/f3′hmutant that does not accumulate anthocyanins were higher than in the wild type. Overall, these results support the hypothesis that photorespiration-related genes might be involved in the regulation of anthocyanin biosynthesis. This finding provides a new insight into our understanding of the mechanism underlying the control of anthocyanin biosynthesis in plants.