Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase.
Long intergenic non-protein coding RNA 02570 promotes nasopharyngeal carcinoma progression by adsorbing microRNA miR-4649-3p thereby upregulating both sterol regulatory element binding protein 1, and fatty acid synthase.
复制标题
长基因间非蛋白编码RNA 02570通过吸附microRNA miR-4649- 3 p从而上调固醇调节元件结合蛋白1和脂肪酸合酶促进鼻咽癌进展。
DOI：
10.1080/21655979.2021.1979317
复制
发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Qu S
中科院分区：
文献类型：
--
作者：
Liu F;Wei J;Hao Y;Lan J;Li W;Weng J;Li M;Su C;Li B;Mo M;Tang F;Wang Y;Yang Y;Jiao W;Qu S
我们前期的研究已经阐明了长基因间非蛋白质编码RNA 2570（LINC 02570）与鼻咽癌（NPC）的可能联系。然而，LINC 02570促进NPC的确切机制仍然未知。我们采用定量聚合酶链反应（qPCR）检测LINC 02570在鼻咽癌细胞系、鼻咽癌组织和慢性鼻炎组织中的表达。亚细胞LINC 02570定位通过荧光原位杂交（FISH）证实。使用3-（4，5-二甲基-2-噻唑基）-2，5-二苯基-2-H-溴化四唑（MTT）、比色焦点形成测定、伤口愈合测定和transwell测定分析LINC 02570稳定敲减和过表达对活力、增殖、迁移和侵袭的影响。RNA串扰分析通过计算机模拟预测了microRNA-4649- 3 p（miR-4649- 3 p）与LINC 02570或固醇调节元件结合转录因子1（SREBF 1）的结合。双荧光素酶报告基因测定用于确认潜在的相互作用。Western blotting法检测固醇调节元件结合蛋白1（SREBP 1）和脂肪酸合成酶（FestivalAcidSynthase，FestivalAcidSynthase）的表达。结果表明，LINC 02570在晚期临床阶段NPC患者中上调，并通过吸附miR-4649- 3 p以上调SREBP 1和FREBP 1来促进NPC进展。本研究阐明了一个潜在的化疗靶点，参与脂质代谢在鼻咽癌。
Our previous studies have elucidated a possible connection between long intergenic non-protein coding RNA 2570 (LINC02570) and nasopharyngeal carcinoma (NPC). However, the precise mechanism by which LINC02570 promotes NPC remains unknown. We used quantitative polymerase chain reaction (qPCR) to detect LINC02570 expression in nasopharyngeal cell lines, NPC tissues, and chronic rhinitis tissues. Subcellular LINC02570 localization was confirmed by fluorescence in situ hybridization (FISH). The effects of LINC02570 stable knockdown and overexpression on viabillity, proliferation, migration, and invasion were analyzed using 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl-2-H-Tetrazolium bromide (MTT), a colorimetric focus-formation assay, a wound healing assay, and transwell assays. RNA crosstalk analysis in silico predicted microRNA-4649-3p (miR-4649-3p) binding to LINC02570 or sterol regulatory element binding transcription factor 1 (SREBF1). A dual luciferase reporter assay was used to confirm potential interactions. Sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FASN) expression were detected by western blotting. The results suggest that LINC02570 is upregulated in late clinical stage NPC patients, and promotes NPC progression by adsorbing miR-4649-3p to up-regulate SREBP1 and FASN. This study elucidates a potential chemotherapeutic target involved in lipid metabolism in NPC.