Sensitive and Selective Measurement of Serotonin in Vivo Using Fast Cyclic Square-Wave Voltammetry.
Sensitive and Selective Measurement of Serotonin in Vivo Using Fast Cyclic Square-Wave Voltammetry.
复制标题
使用快速循环方波伏安法灵敏、选择性地测量体内血清素。
DOI：
10.1021/acs.analchem.9b03164
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发表时间：
2020
影响因子：
7.4
通讯作者：
Jang,DongPyo
中科院分区：
文献类型：
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作者：
Shin,Hojin;Oh,Yoonbae;Park,Cheonho;Kang,Yumin;Cho,HyunU;Blaha,CharlesD;Bennet,KevinE;Heien,MichaelL;Kim,InYoung;Lee,KendallH;Jang,DongPyo
虽然n型快速扫描循环伏安法（N-FSCV）作为一种电分析方法已被广泛应用于体内细胞外血清素浓度的测定，但其灵敏度和选择性都有待提高。基于我们之前使用快速循环方波伏安法（FCSWV）测量体内多巴胺的研究，我们对该技术进行了改进，以优化体内血清素的检测。一系列大振幅方形电位叠加到n形波形上，在n形波形内提供多个氧化还原反应的循环，以提高与二维伏安图结合测量血清素的灵敏度和选择性。n形快速循环方波伏安法（N-FCSWV）对血清素的敏感性显著高于常规N-FSCV。此外，N-FCSWV在区分血清素与其主要干扰物多巴胺和5-羟基吲哚乙酸（5-HIAA）方面表现出比传统n型FSCV更好的性能。大振幅方波不影响局部神经元活动，可监测全身注射羟色胺选择性再摄取抑制剂艾司西酞普兰（ESCIT, 10 mg/kg i.p）前后电刺激引起的大鼠黑质网状部（SNr）血清素相释放。
Although N-shaped fast scan cyclic voltammetry (N-FSCV) is well-established as an electroanalytical method to measure extracellular serotonin concentrationsin vivo, it is in need of improvement in both sensitivity and selectivity. Based on our previous studies using fast cyclic square-wave voltammetry (FCSWV) forin vivodopamine measurements, we have modified this technique to optimize the detection of serotoninin vivo. A series of large amplitude square-shaped potentials was superimposed onto an N-shaped waveform to provide cycling through multiple redox reactions within the N-shaped waveform to enhance the sensitivity and selectivity to serotonin measurement when combined with a two-dimensional voltammogram. N-Shaped fast cyclic square-wave voltammetry (N-FCSWV) showed significantly higher sensitivity to serotonin compared to conventional N-FSCV. In addition, N-FCSWV showed better performance than conventional N-shaped FSCV in differentiating serotonin from its major interferents, dopamine and 5-hydroxyindoleascetic acid (5-HIAA). It was also confirmed that the large amplitude of the square waveform did not influence local neuronal activity, and it could monitor electrical stimulation evoked phasic release of serotonin in the rat substantia nigra pars reticulata (SNr) before and after systemic injection of escitalopram (ESCIT, 10 mg/kg i.p.), a serotonin selective reuptake inhibitor.